FACTORS INVOLVED IN THE ADHERENCE OF CANDIDA-ALBICANS AND CANDIDA-TROPICALIS TO PROTEIN-ADSORBED SURFACES - AN INVITRO STUDY USING IMMOBILIZED PROTEIN
FACTORS INVOLVED IN THE ADHERENCE OF CANDIDA-ALBICANS AND CANDIDA-TROPICALIS TO PROTEIN-ADSORBED SURFACES - AN INVITRO STUDY USING IMMOBILIZED PROTEIN
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DOI：
10.1007/bf00437146
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发表时间：
1992-06-01
期刊：
影响因子：
5.5
通讯作者：
OKUDA, K
中科院分区：
文献类型：
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作者：
NIKAWA, H;SADAMORI, S;OKUDA, K
用血清或唾液蛋白共价结合的表面修饰玻片研究了白色念珠菌和热带念珠菌对蛋白质吸附表面的粘附性。在白色念珠菌和黏液或唾液蛋白固定的玻片之间观察到一种特殊的粘附，如配体-受体相互作用。通过玻片的去糖基化消除了这种相互作用。提示受体可能是一种含有黏液或唾液的低聚糖。在纤维蛋白原固定的玻璃表面上也观察到类似的特异性相互作用。当将去糖基化蛋白固定化玻璃载玻片的贴壁细胞数量与这些蛋白固定化玻璃载玻片的zeta电位和接触角进行对比时，发现白色念珠菌的贴壁细胞数量与zeta电位之间存在显著相关性（r = - 0.87），而热带念珠菌的贴壁细胞数量与接触角之间存在显著相关性（r = 0.82）。这些结果表明，控制真菌粘附到假牙膜的力可能会根据真菌和基质的表面特性而变化。
The adherence of Candida albicans and C. tropicalis to protein-adsorbed Surfaces was investigated with surface-modified glass slides to which serum or salivary proteins were covalently bound. A specific adherence like a ligand-receptor interaction was observed between C. albicans and mucin- or salivary protein-immobilized glass slides. This interaction was eliminated by deglycosylation of the slides. suggesting that the receptor may be an oligosaccharide(s) contained mucin or saliva. A similar specific interaction was also observed between C. tropicalis and fibrinogen-immobilized glass surfaces. When the numbers of adherent cells to deglycosylated protein-immobilized glass glides were plotted against zeta potentials and contact angles of these protein-immobilized glass slides, a significant correaltion was observed between the numbers of adherent cells and zeta potentials in the case of C. albicans (r = - 0.87), whereas a significant correlation was observed between cell numbers and contact angles (r = 0.82) in the case of C. tropicalis. These results suggest that the forces governing the adherence of fungi to pellicle in dentures may vary depending upon thc surface properties of fungi and substrate.