Gas chromatographic analysis of resveratrol in plasma, lipoproteins and cells after in vitro incubations
Gas chromatographic analysis of resveratrol in plasma, lipoproteins and cells after in vitro incubations
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DOI：
10.1016/s0378-4347(97)00383-6
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发表时间：
1997-11-21
期刊：
影响因子：
--
通讯作者：
Loreau, N
中科院分区：
文献类型：
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作者：
Blache, D;Rustan, I;Loreau, N
白藜芦醇是存在于某些红葡萄酒中的三羟基芪。它可能在抑制脂蛋白氧化和血小板活性方面发挥作用。我们已经开发出第一种方法来测量动物和人体样品中的白藜芦醇，并研究其在体外的掺入。加入表前列烷醇作为内标物后，在抗氧化剂存在下并在昏暗的灯光下对样品进行脂质提取，以使反式白藜芦醇至顺式形式的变性和异构化最小化。提取物通过冷丙酮沉淀纯化，含白藜芦醇的丙酮相在氮气下蒸发。白藜芦醇作为三甲基甲硅烷基衍生物进行了分析，通过毛细管气相色谱法解决顺式和反式白藜芦醇（6.6和12.9分钟，分别）。加标纯反式白藜芦醇（0.1至10 μ g）的样品的分析表明，该方法是具体的，并给出了良好的线性和回收率（96.8%），具有较高的重现性（变异系数：3.3%）。检测限约为50 ng/ml。应用表明，白藜芦醇在体外与血浆、脂蛋白和细胞孵育后被掺入血细胞和脂蛋白中。(C)1997年Elsevier Science B.V.
Resveratrol is a trihydroxystilbene present in certain red wines. It may play a role in the inhibition of lipoprotein oxidation and platelet activity. We have developed the first method to measure resveratrol in animal and human samples and to study its incorporation in vitro. After adding epicoprostanol as an internal standard, samples are subjected to lipid extraction in the presence of antioxidant and under dim light to minimize both denaturation and isomerization of the trans-resveratrol to the cis-form. Extracts were purified by cold acetone precipitation and the resveratrol-containing acetone phase was evaporated under nitrogen. The resveratrol was analyzed as a trimethylsilyl derivative by capillary gas chromatography which resolved the cis- and trans-resveratrol (6.6 and 12.9 min, respectively). Analyses of samples spiked with pure trans-resveratrol (0.1 to 10 mu g) indicated that the method was specific and gave excellent linearity and recovery (96.8%) with a high reproducibility (coefficient of variation: 3.3%). The detection limit was about 50 ng/ml. Applications show that resveratrol was incorporated into blood cells and lipoproteins after in vitro incubations with plasma, lipoproteins and cells. (C) 1997 Elsevier Science B.V.