Optimization of anterior eye fluorescein viewing
Optimization of anterior eye fluorescein viewing
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DOI：
10.1016/j.ajo.2006.04.062
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发表时间：
2006-10-01
影响因子：
4.2
通讯作者：
Fowler, Colin W.
中科院分区：
文献类型：
--
作者：
Peterson, Rachael C.;Wolffsohn, James S.;Fowler, Colin W.
中心点目的：优化前眼荧光素观察和图像捕捉。中心点设计：前瞻性实验研究。中心点方法：测量10种不同型号裂隙灯蓝光亮度的光谱辐射和3种屏障滤光片的光谱透过率。通过对10名受试者4种不同的荧光素滴注方法的比较，评价了临床上最佳的荧光素滴注方法。两种方法使用一朵小花，两种使用不同浓度的极小花。对所产生的荧光随时间的猝灭效果和效率进行评估。中心点结果：蓝色照明的光谱辐射通常在460 nm处有一个平均峰值。对同一型号的三个裂隙灯进行比较，得到了相似的光谱辐射分布。在所检查的狭缝灯具中，8.3%至50.6%的照明输出针对80%的荧光素激发进行了优化，1.2%至23.5%的照明与荧光团发射的照明重叠。屏障过滤器的平均截止值为510到520次。所有荧光素滴注方法均观察到猝灭现象。湿润的小花和1%的小花在平均20s内达到了有用的荧光水平(类似于饱和小花和2%的小花的2.5倍快)，持续了大约160秒。中心网点结论：大多数裂隙灯的蓝光和黄色屏障滤光片并不是观察和捕获荧光素的最佳选择。使用湿润的小花或1%微量荧光素滴注在临床上似乎是最合适的，因为较低的荧光素数量和浓度可以提高临床检查的效率。《眼科杂志》2006；142：572-575。(C)2006，Elsevier Inc.保留所有权利。)
center dot PURPOSE: To optimize anterior eye fluorescein viewing and image capture.center dot DESIGN: Prospective experimental investigation.center dot METHODS: The spectral radiance of ten different models of slit-lamp blue luminance and the spectral transmission of three barrier filters were measured. Optimal clinical instillation of fluorescein was evaluated by a comparison of four different instillation methods of fluorescein into 10 subjects. Two methods used a floret, and two used minims of different concentration. The resulting fluorescence was evaluated for quenching effects and efficiency over time.center dot RESULTS: Spectral radiance of the blue illumination typically had an average peak at 460 nm. Comparison between three slit-lamps of the same model showed a similar spectral radiance distribution. Of the slit,lamps examined, 8.3% to 50.6% of the illumination output was optimized for > 80% fluorescein excitation, and 1.2% to 23.5% of the illumination overlapped with that emitted by the fluorophore. The barrier filters had an average cut-off at 510 to 520 run. Quenching was observed for all methods of fluorescein instillation. The moistened floret and the 1% minim reached a useful level of fluorescence in on average similar to 20s (similar to 2.5x faster than the saturated floret and 2% minim) and this lasted for similar to 160 seconds.center dot CONCLUSIONS: Most slit-lamps' blue light and yellow barrier filters are not optimal for fluorescein viewing and capture. Instillation of fluorescein using a moistened floret or 1% minim seems most clinically appropriate as lower quantities and concentrations of fluorescein improve the efficiency of clinical examination. (Am J Ophthalmol 2006; 142:572-575. (c) 2006 by Elsevier Inc. All rights reserved.)