MicroRNA expression profiles of trophoblastic cells
MicroRNA expression profiles of trophoblastic cells
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DOI：
10.1016/j.placenta.2012.05.009
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发表时间：
2012-09-01
期刊：
影响因子：
3.8
通讯作者：
Markert, U. R.
中科院分区：
文献类型：
--
作者：
Morales-Prieto, D. M.;Chaiwangyen, W.;Markert, U. R.
背景：微小RNA（microRNAs，miRNAs）是一类小的单链RNA分子，在基因表达中起着转录后调节剂的作用。滋养层细胞是一组异质性的胎儿细胞，形成母胎界面，并显示出广泛的功能。它们的行为调控可能部分是通过miRNAs进行的。因此，我们的目的是比较不同滋养层细胞系的原代早期和晚期滋养层细胞的miRNA谱。材料和方法：从健康足月和妊娠早期胎盘的分离的细胞滋养层细胞和细胞系HTR-8/SVneo中获得总RNA（永生化滋养层细胞）、JEG-3（绒毛膜癌）、ACH-3 P和AC 1-M59，它们分别是与早期和晚期妊娠滋养层细胞融合的绒毛膜癌细胞。使用TaqMan Human MicroRNA Array定量分析762种不同miRNA的表达水平。结果：所分析的细胞类型具有许多相似的miRNA模式，但在三个miRNA簇的表达上有显著差异：染色体19 miRNA簇（C19 MC;含有54种不同的miRNA）。C14 MC（34个miRNA）和次要簇（miRNA-371至miRNA-373簇），也位于19号染色体上。从早期到晚期，C19 MC内的miRNA表达显著增加，而C14 MC成员的miRNA表达降低。miR-371-3簇内的miRNA略有增加。HTR-8/SVneo细胞不表达C19 MC和miR-371-3簇，而在绒毛膜癌衍生的细胞系中几乎检测不到C14 MC。http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi? acc= GSE 32346）。除了上述簇内的miRNA之外，还有27种miRNA在足月和妊娠早期滋养层细胞之间差异表达（>100倍）。胎盘特异性miRNAs miR-141、miR-21和let-7 g在所有检测细胞中均有表达，其中在原代滋养层细胞中表达最高。结论：早孕期和足月滋养层细胞与滋养层细胞株的miRNAs指纹图谱存在较大差异，这可能与它们不同的行为和特征有关。(C)2012爱思唯尔有限公司保留所有权利。
Background: MicroRNAs (miRNAs) are small single-stranded RNA molecules working as post-transcriptional modulators of gene expression. Trophoblast cells are a heterogenous group of fetal cells forming the feto maternal interface and displaying a wide spectrum of functions. The regulation of their behavior may partly underly the control through miRNAs. Therefore, we aimed to compare the miRNA profile of primary first and third trimester trophoblast cells with that of different trophoblastic cell lines.Material and methods: Total RNA was obtained from isolated cytotrophoblast cells from healthy term and first trimester placentae and the cell lines HTR-8/SVneo (immortalized trophoblast cells), JEG-3 (choriocarcinoma), ACH-3P and AC1-M59, which are choriocarcinoma cells fused with first and third trimester trophoblast cells, respectively. The expression level of 762 different miRNAs was quantitatively analyzed by using a TaqMan Human MicroRNA Array. For testing the reproducibility of the array technique, the expression of 9 selected miRNAs has been re-analyzed by individual qPCR.Results: The analyzed cell types share many similar patterns of miRNAs, but are significantly distinct in the expression of three miRNA clusters: chromosome 19 miRNA cluster (C19MC; containing 54 different miRNAs). C14MC (34 miRNAs) and a minor cluster (miRNA-371 to miRNA-373 cluster), also located on chromosome 19. Expression of miRNAs within C19MC increases significantly from first to third trimester trophoblast while that of C14MC members decreases. MiRNAs within the miR-371-3 cluster augment slightly. C19MC and the miR-371-3 cluster are not expressed by HTR-8/SVneo cells whilst C14MC is almost not detectable in the choriocarcinoma-derived cell lines complete array data available at NCBI Gene Expression Omnibus accession number GSE32346: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32346). Beside the miRNAs within the mentioned clusters, further 27 miRNAs are differentially expressed (>100 fold) between term and first trimester trophoblast cells. The placenta-specific miRNAs miR-141 and miR-21 as well as let-7g are expressed in all tested cells with the highest expression in primary trophoblast cells.Conclusion: Primary first trimester and term trophoblast cells and trophoblastic cell lines display major differences in their miRNA fingerprints which may be involved in their different behavior and characteristics. (C) 2012 Elsevier Ltd. All rights reserved.