Rapid Screening Method for Residual Veterinary Drugs in Meat and Fish by HPLC
Rapid Screening Method for Residual Veterinary Drugs in Meat and Fish by HPLC
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高效液相色谱法快速筛查肉类和鱼类中兽药残留
DOI：
10.3358/shokueishi.39.6_383
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Nakazawa
中科院分区：
文献类型：
--
作者：
M. Horie;Terumitsu Yoshida;Koichi Saito;H. Nakazawa
建立了一种同时测定肉和鱼中13种兽药(土霉素、喹恶啉-2-甲酸、噻苯咪唑、5-羟基噻苯咪唑、阿苯达唑-2-氨基磺酸、磺胺嘧啶、氟苯达唑、玉米赤霉醇、β-曲诺酮、磺胺二甲氧星、磺胺间甲氧西林、恶灵和恩诺沙星)的高效液相色谱快速筛选方法。用0.25%偏磷酸-甲醇-乙腈(6：2：2，v/v)提取，用OASIS HLB60 mg小柱净化。色谱柱为Wakosil-II-5C18 RS柱(150×4.6 mm i.d.)以0.025mol/L磷酸盐缓冲液(pH 2.5)-乙腈为流动相，流速0.6mL/min。用紫外光(UV)和荧光检测器按时间编程进行检测。每种药物在1~20 ng范围内线性关系良好。在0.020.1μg/g范围内，回收率为75.1%~90.5%。
A simple and rapid screening method using HPLC for the simultaneous determination of 13 veterinary drugs (oxytetracycline, quinoxaline-2-carboxylic acid, thiabendazole, 5-hydroxythiabendazole, albendazole-2-aminosulfone, sulfadimidine, flubendazole, zeranol, β-trenbolone, sulfadimethoxine, sulfamonomethoxine, oxolinic aid, and enrofloxacin) in meat and fish has been developed. The drugs were extracted with 0.25% metaphosphoric acidmethanol-acetonitrile (6:2:2, v/v), and the extracts were cleaned up on an OASIS HLB (60mg) cartridge. The HPLC separation was performed on a Wakosil-II-5C18 RS column (150×4.6mm i. d.) with a gradient system of 0.025mol/L phosphate buffer (pH 2.5)-acetonitrile as the mobile phase at a flow rate of 0.6mL/min. The drugs were detected with ultraviolet (UV) and fluorescence detectors with time programming. The calibration graphs were rectilinear from 1 to 20ng for each drug. The recoveries at levels of 0.02-0.1μg/g were 75.1-90.5%.