Remodeling of Buccal Mucosa by Bladder Microenvironment
Remodeling of Buccal Mucosa by Bladder Microenvironment
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DOI：
10.1016/j.urology.2009.12.060
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发表时间：
2010-06-01
期刊：
影响因子：
2.1
通讯作者：
Cao, Yilin
中科院分区：
文献类型：
--
作者：
Lu, Mujun;Zhou, Guangdong;Cao, Yilin
目的探讨膀胱黏膜重建的最佳细胞来源。方法将猪口腔黏膜移植到自体膀胱黏膜缺损处，分别于术后3、6、12个月用免疫组织化学和RT-PCR方法检测口腔黏膜和膀胱尿路上皮细胞的特异性标志物表达，以检测口腔黏膜向膀胱尿路上皮细胞表型转化的可能性。结果移植后3个月内，口腔黏膜细胞角蛋白14(CK14)阳性表达，尿路上皮特异性标志物UPII阴性表达。然而，移植后6个月，移植物在蛋白和mRNA水平均表达UPII，并在术后12个月保持表型。结论首次揭示了口腔角质形成细胞向尿路上皮细胞的跨分化潜能，以及膀胱局部微环境在口腔黏膜上皮重建中的重要作用。这些结果支持口腔角质形成细胞可以作为重建膀胱粘膜的潜在细胞来源的假说。泌尿外科75：1514.e7-1514.e14,2010年。(C)2010年爱思唯尔公司。
OBJECTIVES The optimal cell source for bladder mucosa reconstruction is in question. This study explored the feasibility of phenotype transformation of oral mucosa towards bladder urothelium by transplanting the oral mucosa into bladder local microenvironment.METHODS Porcine oral mucosa grafts were transplanted into autologous bladder mucosa defects, and the specific marker expressions of both oral epithelium and bladder urothelium were examined by immunohistochemistry and RT-PCR at 3, 6, and 12 months to detect a potential phenotype transformation.RESULTS The grafts could retain the phenotype and structure of oral mucosa within 3 months with positive expression of cytokeratin 14 (CK 14, a specific marker of oral epithelium) and negative expression of uroplakin II (UPII, a urothelium-specific marker). However, after 6 months of transplantation, the grafts expressed UPII at both protein and mRNA levels and the phenotype persisted at 12 months postsurgery. All the grafts continuously retained the positive expression of CK 14 at all time points.CONCLUSIONS These findings, for the first time, revealed the transdifferential potential of oral keratinocytes toward urothelial cells and the important role of bladder local microenvironment for remodeling oral mucosa epithelium. These results support the hypothesis that oral keratinocytes can serve as a potential cell source for reconstructing bladder mucosa. UROLOGY 75: 1514.e7-1514.e14, 2010. (c) 2010 Elsevier Inc.