Ultrastructural study of pancreatic B cell regeneration in newborn rats after destruction by streptozotocin
Ultrastructural study of pancreatic B cell regeneration in newborn rats after destruction by streptozotocin
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链脲佐菌素破坏新生大鼠胰腺B细胞再生的超微结构研究
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
É. Hollande
中科院分区：
文献类型：
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作者：
M. C. Dutrillaux;B. Portha;C. Rozé;É. Hollande
对出生当天单次注射链脲佐菌素的大鼠胰腺内分泌细胞的超微结构进行了研究。大多数出生时存在的B细胞被链脲佐菌素破坏，但也有一些存活下来，并在消除吞噬溶酶体结构中改变的片段后再次合成胰岛素。新的B细胞主要是通过小胰管形成新的胰岛而产生的。秋水仙素处理的动物的有丝分裂研究表明，第4天出现的大多数B细胞是由未分化细胞的有丝分裂形成的。在链脲佐菌素处理的大鼠中，没有显示出预先存在的分化的B细胞的显著分裂。因此，这个新生大鼠模型中的B细胞再生主要是通过从导管中萌发新的胰岛来解释的。
SummaryAn ultrastructural study of endocrine cells was performed in the pancreas of rats treated with a single dose of streptozotocin on the day of birth. Most of the B cells present at birth were destroyed by streptozotocin but some survived and could again synthesize insulin after eliminating their altered fragments in phago-lysosome structures. New B cells were produced primarily by the formation of new islets from the small pancreatic ducts. A study of mitosis in colchicine treated animals showed that most B cells present on day 4 were formed by mitosis of undifferentiated cells. No significant division of preexisting differentiated B cells could be shown in streptozotocin treated rats.B cell regeneration in this newborn rat model is thus explained primarily by budding of new islets from the ducts.