Anti-allergic activity of R-phycocyanin from Porphyra haitanensis in antigen-sensitized mice and mast cells
Anti-allergic activity of R-phycocyanin from Porphyra haitanensis in antigen-sensitized mice and mast cells
复制标题
DOI：
10.1016/j.intimp.2015.02.032
复制
发表时间：
2015-04-01
影响因子：
5.6
通讯作者：
Liu, Guangming
中科院分区：
文献类型：
--
作者：
Liu, Qingmei;Wang, Youzhao;Liu, Guangming
在亚洲国家，食物过敏的流行率有所增加。海藻已被认为是抗过敏药物的潜在来源。本研究旨在从坛紫菜中分离出R-藻蓝蛋白(RPC)，并检测其在抗原致敏小鼠和肥大细胞中的抗过敏作用。在动物实验中，RPC能有效降低原肌球蛋白(TM)特异性免疫球蛋白E(IgE)和组胺水平，减轻小鼠过敏症状和空肠组织炎症，并抑制腹腔灌洗液中细胞因子(IL-4和IL-13)的表达和释放。在脾淋巴细胞实验中，高纯度的RPC使CD4(+)T细胞的免疫功能向Th1活性倾斜。TM和RPC通过Jun氨基末端激酶和Janus激酶2信号通路协同作用诱导干扰素-γ的高表达，RPC和TM联合诱导干扰素-γ的合成。在IgE介导的大鼠肥大细胞RBL-2H3上评价RPC的抗过敏作用。结果表明，RPC可抑制抗原致敏的RBL-2H3细胞的过敏标志物，包括β-己糖苷酶、组胺和ROS的释放。RPC还抑制促炎因子(IL-4和肿瘤坏死因子-α)的产生。总之，RPC通过阻断Th2细胞极化降低了对TM的变态反应敏感性，并抑制了抗原刺激的肥大细胞中变态反应介质的释放。可作为过敏症患者的功能性食品成分或活性药物成分。(C)2015爱思唯尔B.V.保留所有权利。
The prevalence of food allergy has increased in Asian countries. Marine algae have been proposed as the potential resource for anti-allergic therapeutics. The present study was aimed at isolating R-phycocyanin (RPC) from Porphyra haitanensis and determining the anti-allergy potential of RPC in antigen-sensitized mice and mast cells. In animal experiments, RPC could effectively reduce tropomyosin (TM)-specific immunoglobulin E (IgE) and histamine levels, alleviate allergy symptoms and jejunum tissue inflammation in mice, and inhibit the expression and release of cytokines (interleukin-4 (IL-4) and IL-13) in peritoneal lavage fluid. In spleen lymphocyte experiments, high purity of RPC skewed the immunological function of CD4(+) T cells towards Th1 activity. A higher expression of interferon (IFN)-gamma was induced by a synergistic effect of TM and RPC Through the Jun N-terminal kinase and Janus kinase 2 signaling pathways, IFN-gamma synthesis was induced by RPC in combination with TM. Anti-allergic effect of RPC was evaluated in IgE-mediated rat mast RBL-2H3 cells. The results demonstrated that RPC inhibited allergy markers, including the release of beta-hexosaminidase, histamine and ROS in antigen-sensitized RBL-2H3 cells. RPC also suppressed the production of pro-inflammatory factors (IL-4 and tumor necrosis factor-alpha). In conclusion, RPC decreased allergic sensitization against TM by blocking Th2 cell polarization as well as suppressed the release of allergic-mediators in antigen-stimulated mast cells. It may be used as a functional food component or active pharmaceutical ingredient for allergic patients. (C) 2015 Elsevier B.V. All rights reserved.