Transcriptome sequencing reveals the effects of cadmium toxicity on the cold tolerance of the wolf spider Pirata subpiraticus
Transcriptome sequencing reveals the effects of cadmium toxicity on the cold tolerance of the wolf spider Pirata subpiraticus
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DOI：
10.1016/j.chemosphere.2020.126802
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发表时间：
2020-09-01
期刊：
影响因子：
8.8
通讯作者：
Song, Qisheng
中科院分区：
文献类型：
--
作者：
Lv, Bo;Wang, Juan;Song, Qisheng
蜘蛛是稻田害虫的主要捕食者，长期暴露于镉污染中。蜘蛛越冬期的适居性与春季种群生长密切相关，但Cd对蜘蛛低温硬度存活的影响及其潜在机制尚不清楚。本研究发现，镉胁迫下狼蛛（Pirata subpiraticus）的一些生长参数（体长、宽度、质量和宜居性）发生了明显的变化。为了在分子水平上研究Cd毒性对蜘蛛的影响，对环境温度变化的蜘蛛进行了rna测序。转录组数据显示，在比较中共产生807个差异表达基因（deg）。获得的deg主要与代谢相关过程相关，包括氧化还原酶活性和脂质转运，25个deg与报道的冷冻保护剂相关，包括甘油、精氨酸、半胱氨酸、热休克蛋白、葡萄糖和甘露糖。生长因子（胰岛素生长因子、血小板源性生长因子和转化生长因子）和细胞色素P450编码基因在蜘蛛体内显著表达。此外，根据转录组学特征对转录因子家族进行了表征，ZBTB转录因子在表征基因中表现出最明显的变化。综上所述，我们的研究表明，Cd对下海蜘蛛在寒冷环境温度下的生长有不利影响，蜘蛛能够通过表达参与能量物质、低温保护剂和免疫相关成分代谢的基因来应对不利的Cd胁迫。(C) 2020 Elsevier Ltd.版权所有。
As the predominant predator of pests in rice fields, spiders have been exposed to cadmium (Cd) pollution for a long time. The livability of spiders during the overwintering period is closely related to population growth in spring, but the effects of Cd on spider's survival of cold hardness and the underlining mechanism remain unclear. In the present study, we found that some growth parameters (body length, width, mass and livability) in the wolf spider Pirata subpiraticus were altered distinctively under Cd stress. To investigate the effects of Cd toxicity on the spider at molecular levels, RNA-sequencing was performed on the spiderlings undergoing ambient temperature alterations. Transcriptome data showed that a total of 807 differentially expressed genes (DEGs) were yielded in the comparison. The obtained DEGs were mainly linked with metabolism-related process, including oxidoreductase activity and lipid transport, and 25 DEGs were associated with the reported cryoprotectants, including glycerol, arginine, cysteine, heat shock protein, glucose and mannose. Growth factors (insulin growth factor, platelet-derived growth factor and transforming growth factor) and cytochrome P450 encoding genes were dramatically expressed in the spider. Furthermore, transcriptional factors (TFs) family were characterized according to the transcriptomic profile, and ZBTB TFs were represented the most distinctive alterations in the characterized genes. Collectively, our study illustrated that Cd poses disadvantageous effects on the growth of P. subpiraticus at cold ambient temperature, and the spiders are capable of responding to the adverse Cd stress by expressing the genes involved in the metabolism of energy substances, cryoprotectants and immune-related components. (C) 2020 Elsevier Ltd. All rights reserved.