Epithelial re-organization and dynamics of progression through mitosis in Drosophila separase complex mutants
Epithelial re-organization and dynamics of progression through mitosis in Drosophila separase complex mutants
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DOI：
10.1242/jcs.01663
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发表时间：
2005-02-15
影响因子：
4
通讯作者：
Lehner, CF
中科院分区：
文献类型：
--
作者：
Pandey, R;Heidmann, S;Lehner, CF
分离酶切割粘连蛋白复合体的一个亚基，从而促进有丝分裂和减数分裂期间姐妹染色单体的分离。果蝇分离酶与pimples和three rows基因编码的调节亚基相关。三排和丘疹，果蝇安全蛋白，是有丝分裂时姐妹染色单体分离所必需的。芽殖酵母分离酶除了提供粘连蛋白亚基切割之外还提供其他功能，这些功能是退出有丝分裂期间纺锤体组织和时间调节所需的。因此，使用活胚胎的延时成像，我们仔细分析了丘疹和三排突变体的有丝分裂进程。我们证明，尽管姐妹染色单体分离完全失败，但这些突变体的表皮细胞退出有丝分裂（包括完整的胞质分裂）仅发生轻微的时间延迟。然而，有趣的是，上皮组织中的明显缺陷在以下间期发展，表明分离酶复合物不仅对遗传稳定性很重要，而且可能间接地对上皮完整性很重要。
Separase cleaves a subunit of the cohesin complex and thereby promotes sister chromatid separation during mitotic and meiotic divisions. Drosophila separase associates with regulatory subunits encoded by the pimples and three rows genes. Three rows and Pimples, the Drosophila securin, are required for sister chromatid separation during mitosis. Budding yeast separase provides other functions in addition to cohesin subunit cleavage, which are required for spindle organization and temporal regulation during exit from mitosis. Therefore, using time-lapse imaging in live embryos, we have carefully analyzed progression through mitosis in pimples and three rows mutants. We demonstrate that despite the total failure of sister chromatid separation, exit from mitosis, including a complete cytokinesis, proceeds with only a minor temporal delay in the epidermal cells of these mutants. Interestingly, however, pronounced defects in the epithelial organization develop in the following interphase, indicating that the separase complex is not only important for genetic stability but also and perhaps indirectly for epithelial integrity.