The Effect of Fluorhydroxyapatite-derived Fluoride on Acid Production by Streptococci
The Effect of Fluorhydroxyapatite-derived Fluoride on Acid Production by Streptococci
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DOI：
10.1177/00220345950740091901
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发表时间：
1995-09
影响因子：
7.6
通讯作者：
N. Guha-Chowdhury;Y. Iwami;T. Yamada;E. Pearce
中科院分区：
文献类型：
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作者：
N. Guha-Chowdhury;Y. Iwami;T. Yamada;E. Pearce
研究了氟羟磷灰石（FHAp）矿物中的氟化物在有氧和严格厌氧条件下对细菌糖酵解的影响，以验证这种矿物可用作牙菌斑中氟化物的储存库的说法。为了将氟化物对细菌糖酵解的直接影响与也从矿物中溶解的羟基或磷酸根离子的间接pH缓冲作用分离，我们通过手动添加KOH或HCl来平衡反应的pH下降时间过程。这确保了pH对糖酵解的影响最小化。在受控的pH下降和严格厌氧条件下，源自含有超过30，100 ppm氟化物的FHAp的溶解的氟化物（即，当矿物中的OH被F取代大于80%时）对变形链球菌的乳酸产生具有直接抑制作用。在自由pH值下降和严格厌氧条件下，增加FHAp中的氟化物含量（开始低至2000 ppm氟化物）似乎对乳酸产生具有显着的间接抑制作用。这可能是通过降低pH缓冲效应的矿物介导的。即使在高氟FHAp的存在下，只有0.01至0.025 mmol/L的氟被发现在反应混合物中，一个可能的结果，非化学计量溶解的FHAp。尽管如此低水平的氟化物，对细菌糖酵解的显着抑制作用被证明。本研究结果表明，高氟FHAp可能作为一个水库的氟抑制厌氧产酸的S。变种人
The effect of fluoride derived from fluorhydroxyapatite (FHAp) minerals on bacterial glycolysis under aerobic and strictly anaerobic conditions was studied to validate the claims that this mineral could be used as a reservoir of fluoride in plaque. To isolate the direct effect of fluoride on bacterial glycolysis from that of an indirect pH-buffering effect of hydroxyl or phosphate ions which are also dissolved from the mineral, we equalized the pH-fall time course of reactions by manually adding KOH or HCl. This ensured that pH effects on glycolysis were minimized. Under controlled pH-fall and strictly anaerobic conditions, fluoride derived from the dissolution of FHAp containing more than 30,100 ppm fluoride (i.e., when the substitution of OH by F in the mineral was greater than 80%) had a direct inhibitory effect on lactic acid production in Streptococcus mutans. Under free pH-fall and strictly anaerobic conditions, increasing amounts of fluoride in FHAp (starting as low as 2000 ppm fluoride), appeared to have a pronounced indirect inhibitory effect on lactic acid production. This was probably mediated through a reducing pH buffer effect of the mineral. Even in the presence of high-fluoride FHAp, only 0.01 to 0.025 mmol/L fluoride was found in the reaction mixtures, a probable result of non-stoichiometric dissolution of FHAp. In spite of such low levels of fluoride, marked inhibitory effects on bacterial glycolysis were demonstrated. The results of this study suggest that high-fluoride FHAp may serve as a reservoir of fluoride for the inhibition of anaerobic acid production by S. mutans.