Determination of minimum inhibitory concentrations
Determination of minimum inhibitory concentrations
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DOI：
10.1093/jac/48.suppl_1.5
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发表时间：
2001-07-01
影响因子：
5.2
通讯作者：
Andrews, JM
中科院分区：
文献类型：
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作者：
Andrews, JM
最低抑菌浓度（MICs）定义为一种抗菌剂在过夜培养后能抑制微生物可见生长的最低浓度，而最低杀菌浓度（MBCs）是指抗菌剂在转种到无抗生素培养基上后能阻止微生物生长的最低浓度。诊断实验室主要使用MIC来确认耐药性，但最常见的是作为一种研究工具来确定新型抗菌剂的体外活性，此类研究的数据已被用于确定MIC折点。MBC的测定频率较低，其主要用途是针对心内膜炎患者血液中的分离株。本文描述了测定MIC和MBC的标准化方法。像所有标准化程序一样，使用者必须遵循该方法，不得擅自修改。该方法提供了有关标准抗生素粉末的储存、储备抗生素溶液的制备、培养基、接种物的制备、培养条件以及结果的读取和解释等信息。还提供了给出对照NCTC和ATCC菌株预期MIC范围的表格。
Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation, and minimum bactericidal concentrations (MBCs) as the lowest concentration of antimicrobial that will prevent the growth of an organism after subculture on to antibiotic-free media. MiCs are used by diagnostic laboratories mainly to confirm resistance, but most often as a research tool to determine the in vitro activity of new antimicrobials, and data from such studies have been used to determine MIC breakpoints. MBC determinations are undertaken less frequently and their major use has been reserved for isolates from the blood of patients with endocarditis. Standardized methods for determining MiCs and MBCs are described in this paper. Like all standardized procedures, the method must be adhered to and may not be adapted by the user. The method gives information on the storage of standard antibiotic powder, preparation of stock antibiotic solutions, media, preparation of inocula, incubation conditions, and reading and interpretation of results. Tables giving expected MIC ranges for control NCTC and ATCC strains are also supplied.