Influence of vitamin D3, 1,25-dihydroxyvitamin D3, and 24,25-dihydroxyvitamin D3 on parathyroid hormone secretion, adenosine 3',5'-monophosphate release, and ultrastructure of parathyroid glands in organ culture.
Influence of vitamin D3, 1,25-dihydroxyvitamin D3, and 24,25-dihydroxyvitamin D3 on parathyroid hormone secretion, adenosine 3',5'-monophosphate release, and ultrastructure of parathyroid glands in organ culture.
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维生素D3、1,25-二羟基维生素D3和24,25-二羟基维生素D3对器官培养中甲状旁腺激素分泌、3,5-单磷酸腺苷释放和甲状旁腺超微结构的影响。
DOI：
10.1210/endo-105-1-237
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发表时间：
1979
期刊：
影响因子：
4.8
通讯作者：
E. Altenähr
中科院分区：
文献类型：
--
作者：
M. Dietel;G. Dorn;R. Montz;E. Altenähr
研究了超生理浓度和生理浓度的维生素D_3、1，25-二羟维生素D_3 [1，25（OH）_2D_3]和24，25-二羟维生素D_3 [24，25（OH）_2D_3]对器官培养的正常猪甲状旁腺和人甲状旁腺腺瘤内分泌活性的影响。在培养基中，甲状旁腺激素（PTH）和cAMP的放射免疫测定，并与组织的超微结构变化。在与1，25（OH）2D 3（范围，0.03-40.0 ng/ml）孵育期间，PTH分泌均匀地从100%降至平均60%。在剂量反应实验中，0.015 ng/ml 1，25（OH）2D 3是引起显著抑制的最低浓度。在消除这种类固醇后，PTH分泌在2小时内再次增加至接近正常值。cAMP释放呈平行变化。维生素D_3（4000 ng/ml）和24，25（OH）_2D_3（40.0和5.0 ng/58：44 ml）无影响。通过与[75 Se]蛋氨酸孵育来检查分泌的PTH的从头合成;其生物学效价是确定的。
The influence of supraphysiological and physiological concentrations of vitamin D3, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], and 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] on the endocrine activity of normal porcine parathyroid glands and human parathyroid adenomas in organ culture was studied. In the medium, parathyroid hormone (PTH) and cAMP were measured radioimmunologically and correlated with ultrastructural changes of the tissue. During incubations with 1,25(OH)2D3 (range, 0.03–40.0 ng/ml), PTH secretion decreased uniformly from 100% to an average of 60%. In a dose-response experiment, 0.015 ng/ml 1,25(OH)2D3 was the lowest concentration causing a significant inhibition. After elimination of this steroid, PTH secretion increased to nearly normal values again within 2 h. cAMP release showed parallel changes. Vitamin D3 (4000 ng/ml) and 24,25(OH)2D3 (40.0 and 5.0 ng/58:44ml) had no effect. De novo synthesis of the secreted PTH was checked by incubation with [75Se]methionine; its biological potency was es...