Size of cells collected from normal human subjects using contact lens cytology.
Size of cells collected from normal human subjects using contact lens cytology.
复制标题
使用隐形眼镜细胞学从正常人类受试者中收集的细胞大小。
DOI：
10.1097/00006324-199705000-00023
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Wilson,G
中科院分区：
文献类型：
--
作者：
Laurent,J;Wilson,G
本文描述了如何使用软性隐形眼镜从角膜上皮表面采集细胞。该程序称为隐形眼镜细胞学检查 (CLC)。通过冲洗从软隐形眼镜上取出细胞并用吖啶橙染色。描述了两种测量细胞大小的方法。首先，使用计算机辅助技术测量细胞大小，该技术根据细胞的轮廓计算细胞的面积。第二种方法更简单，因为它只需要对电池的最长尺寸（电池长度）进行一次测量。为了测试这种更简单方法的有效性，将 185 个细胞的细胞面积与细胞长度进行了比较。由此产生的相关性（r = 0.92）表明，可以使用细胞长度代替更耗时的细胞面积测量来充分描述脱落细胞的大小。可以使用数学关系将细胞长度转换为细胞面积，以便可以比较实验者使用不同尺寸测量的结果。当 CLC 收集的大量细胞被分成四份并由两名观察者在不同的两天测量细胞长度时，观察者或日期之间没有显着差异。因此，该技术不依赖于一名观察者，并且不受 24 小时测量延迟的影响。从正常人类受试者的角膜上皮中收获细胞。从隐形眼镜的任何一次摘除中收集的细胞数量范围为 10 至 175 个细胞，平均值为 66.8+/-40.4 (N= 46)。测量每个受试者双眼的细胞长度并绘制为频率直方图。细胞长度的范围为10至80μm。个体受试者的平均细胞长度的下限为26.5+/-9.0μm，上限为44.2+/-10.2μm，所有右眼的总平均值为36.0+/-5.1μm，所有左眼的总平均值为34.6+/-5.2μm。所有眼睛的平均值为35.3+/-5.1μm。使用 23 只右眼（N = 1310 个细胞）和 23 只左眼（N = 1765 个细胞）的组合数据创建复合直方图。单个直方图和复合直方图不呈正态分布。分布中的峰值表明存在不同的细胞亚群，这支持了细胞脱落有不止一种机制的假设。（Optom Vis Sci 1997；74：280-287）
This paper describes how a soft contact lens can be used to harvest cells from the surface of the corneal epithelium. The procedure is called contact lens cytology (CLC). Cells were removed from a soft contact lens by irrigation and stained with acridine orange. Two methods for the measurement of cell size are described. First, cell size was measured using a computer-assisted technique, which calculated the area of the cell from its outline. The second method was simpler in that it required only a single measurement of the longest dimension of the cell (the cell length). To test the validity of this simpler method, cell area was compared with cell length in 185 cells. The resulting correlation (r= 0.92) suggests that the size of shed cells can be described adequately using cell length in place of the more time-consuming measurement of cell area. A mathematical relation can be used to convert cell length to cell area so that results from experimenters using different measures of size can be compared. When a large pool of cells collected by CLC was divided into four aliquots and cell length measured by two observers on two different days, there were no significant differences between observers or days. Thus, the technique does not depend on one observer, and it is unaffected by a 24-h delay in measurement. Cells were harvested from the corneal epithelium of normal human subjects. The number of cells collected from any single removal of the contact lens had a range of 10 to 175 cells, and a mean of 66.8+/-40.4 (N= 46). Cell length was measured and plotted as frequency histograms for both eyes of each subject. The range in cell length was from 10 to 80 [mu] m. The mean cell length for individual subjects had a low of 26.5+/-9.0 [mu] m and a high of 44.2+/-10.2 [mu] m, with a grand mean for all right eyes of 36.0+/-5.1 [mu] m, and a grand mean for all left eyes of 34.6+/-5.2 [mu] m. The mean for all eyes was 35.3+/-5.1 [mu] m. Composite histograms were created with the combined data from the 23 right eyes (N= 1310 cells), and the 23 left eyes (N= 1765 cells). Individual histograms and the composite histograms were not normally distributed. Peaks in the distributions suggest the presence of different subpopulations of cells, lending support to the hypothesis that there is more than one mechanism for cell shedding.(Optom Vis Sci 1997; 74: 280-287)