Heterogeneity of glycine receptors and their messenger RNAs in rat brain and spinal cord.
Heterogeneity of glycine receptors and their messenger RNAs in rat brain and spinal cord.
复制标题
大鼠脑和脊髓中甘氨酸受体及其信使 RNA 的异质性。
DOI：
10.1126/science.2845580
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Miledi,R
中科院分区：
文献类型：
--
作者：
Akagi,H;Miledi,R
从成年或新生大鼠脊髓中分离的信使RNA在蔗糖梯度中分级。将这些组分注射到异种卵母细胞中，以确定它们表达甘氨酸受体（GlyRs）的效力，然后进行电生理学检查。沉降曲线揭示了两类GlyR mRNA，一类重，另一类轻。成年脊髓富含重GlyR mRNA，而轻GlyR mRNA在新生儿脊髓和成年大脑皮质中更丰富。成年脊髓重、轻链甘氨酸受体mRNA编码的甘氨酸受体具有一定的电生理差异。因此，有两种类型的GlyR编码的mRNA的大小不同，这些mRNA的表达是发育调节。异质性GlyR mRNA的组织和年龄依赖性分布可能意味着GlyR在中枢神经系统神经元功能中的不同作用。
Messenger RNAs isolated from adult or newborn rat spinal cord were fractionated in a sucrose gradient. The fractions were injected intoXenopusoocytes to determine their potencies for expression of glycine receptors (GlyRs), which were then examined electrophysiologically. The sedimentation profiles disclosed two classes of GlyR mRNAs, one heavy and the other light. The adult spinal cord was rich in heavy GlyR mRNA, whereas the light GlyR mRNA was more abundant in neonatal spinal cord and in adult cerebral cortex. Glycine receptors encoded by heavy and light mRNAs of adult spinal cord showed some electrophysiological differences. Thus there are two types of GlyRs encoded by mRNAs of different sizes, and the expression of these mRNAs is developmentally regulated. A tissue- and age-dependent distribution of heterogeneous GlyR mRNAs may imply diverse roles of the GlyRs in neuronal function in the central nervous system.