Significant changes in ITIH4, AHSG, ORM1, and CD46 content in milk fat globule membrane proteins of ketotic dairy cows
Significant changes in ITIH4, AHSG, ORM1, and CD46 content in milk fat globule membrane proteins of ketotic dairy cows
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酮症奶牛乳脂肪球膜蛋白ITIH4、AHSG、ORM1、CD46含量显着变化
DOI：
10.1017/s0022029917000644
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发表时间：
2017-11-01
影响因子：
2.1
通讯作者：
Xu, Chuang
中科院分区：
文献类型：
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作者：
Gao, Sansi;Yang, Wei;Xu, Chuang
奶牛酮症引起的血液中高浓度的非酯化脂肪酸（NEFA）和-羟基丁酸（BHBA）与炎症状态有关。我们假设，产后奶牛的酮症不仅会导致血浆中炎症相关蛋白的水平改变，还会导致乳脂球膜（MFGM）中的炎症相关蛋白水平改变。从中国黑龙江的一个奶牛场挑选了30头奶牛。采用ELISA试剂盒检测炎性乳脂球膜蛋白，全自动生化分析仪检测血浆BHBA、NEFA、葡萄糖（GLU）、甘油三酯（TG）浓度。与非酮症奶牛相比，酮症奶牛乳中MFGM蛋白中急性期反应蛋白（补体C3 （C3）、凝血酶原（F2）、α -1-酸性糖蛋白（ORM1）、α -胰蛋白酶间抑制剂重链H4 （ITIH4）、α -2- hs -糖蛋白（AHSG）、补体C9 （C9）、补体调节蛋白变体4 (CD46)）的含量显著不同。血中C3、补体C9 （C9）、肿瘤坏死因子（TNF）、MFGM C3、单核细胞分化抗原CD14 （CD14）和ORM1水平与能量平衡相关。在酮症发病前，ITIH4和CD46升高，AHSG和ORM1降低。这些生物标记物为高危奶牛酮症的预测和监测提供了潜力。
High concentrations of non-esterified fatty acid (NEFA) and -hydroxybutyrate (BHBA) in cows' blood caused by ketosis are associated with inflammatory states. We hypothesised that ketosis in postparturient dairy cows would result in altered levels on inflammation-related proteins not only in plasma but also in the milk fat globule membranes (MFGM). Thirty cows were selected from a dairy farm in Heilongjiang, China. Inflammatory milk fat globule membrane proteins were detected using ELISA kits, and a fully automatic biochemical analyser was used to measure the concentrations of BHBA, NEFA, glucose (GLU) and triglyceride (TG) in plasma. MFGM protein from milk of ketotic cows contained significantly different concentrations of acute-phase response proteins (complement C3 (C3), prothrombin (F2), alpha-1-acid glycoprotein (ORM1), inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), alpha-2-HS-glycoprotein (AHSG), complement C9 (C9), complement regulatory protein variant 4 (CD46)) in comparison with milk from non-ketotic cows. Blood concentrations of C3, complement C9 (C9), tumour necrosis factor (TNF), MFGM C3, monocyte differentiation antigen CD14 (CD14) and ORM1 levels were correlated with energy balance. ITIH4 and CD46 increased, and AHSG and ORM1 decreased before the onset of ketosis. These biomarkers offer potential as predictors and monitors of ketosis in at-risk cows.