Assessment of the serodiagnostic potential of nine novel proteins from Mycobacterium tuberculosis.
Assessment of the serodiagnostic potential of nine novel proteins from Mycobacterium tuberculosis.
复制标题
评估结核分枝杆菌九种新型蛋白质的血清诊断潜力。
DOI：
10.1111/j.1574-6968.2001.tb10615.x
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发表时间：
2001
影响因子：
2.1
通讯作者：
Nano,FE
中科院分区：
文献类型：
--
作者：
Moran,AJ;Treit,JD;Whitney,JL;Abomoelak,B;Houghton,R;Skeiky,YA;Sampaio,DP;Badaró,R;Nano,FE
To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins ofMycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulmonary tuberculosis (EP-TB) or HIV-positive individuals with pulmonary tuberculosis (TBH). Specific and high level antibody responses were obtained for four recombinant proteins, of which antigen GST-822 was recognized by 60% of EP-TB and 42% of TBH and antigen MBP-506 was recognized by 45% of EP-TB and 61% of TBH. These results suggest that these proteins are strong candidates as subunits in a polyvalent serodiagnostic test.
