Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios.
Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios.
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DOI：
10.7150/jca.17747
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发表时间：
2017
影响因子：
3.9
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Shang S;Li W;Qin X;Zhang S;Liu Y
异常岩藻糖基化在调节个体发育和细胞分化中起功能性作用，并且在癌症状况中受到差异调节，这可以为癌症诊断和监测提供标志。我们以前开发了一种基于磁珠的凝集素ELISA系统来测量岩藻糖基化触珠蛋白（Hp），这已被报道是通过一系列糖蛋白组学分析的癌症生物标志物。在这项研究中，血清岩藻糖基化Hp的比例，使用我们的ELISA试剂盒在一个单独的队列中的260例患者独立，包括130名健康对照和130例肝细胞癌（HCC）患者。分别计算岩藻糖基化Hp /Hp比值（岩藻糖基化Hp水平/Hp蛋白水平）和ELISA指数（岩藻糖基化Hp OD值/Hp蛋白OD值），以反映其蛋白水平上的Hp岩藻糖基化水平。我们的数据显示，岩藻糖基化Hp /Hp比值（AUC=0.8449）和ELISA指数（AUC=0.8581）在区分HCC和对照组方面具有更好的性能，这表明岩藻糖基化Hp比值即使在低甲胎蛋白（AFP）水平下也可以提高HCC的诊断和预测。此外，AFP和岩藻糖基化Hp比值的组合分析增加了HCC诊断的AUC值。
Aberrant fucosylation plays a functional role in regulating ontogeny and celluar differentiation and are differentially regulated in cancerous condition, which could provide hallmarks for cancer diagnostics and surveillance. We previously developed a magnetic beads-based lectin ELISA system to measure fucosylated haptoglobin (Hp), which has been reported to be a cancer biomarker through a series of glycoproteomic analysis. In this study, serum fucosylated Hp ratios were measured using our ELISA kit in a separate cohort of 260 patients independently, including 130 healthy controls and 130 patients with hepatocellular carcinoma (HCC). Fucosylated Hp /Hp ratio (levels of fucosylated Hp /levels of protein Hp) and ELISA Index (OD value of fucosylated Hp /OD value of protein Hp) were calculated respectively to reflect Hp fucosylation level on its protein level. Our data showed that fucosylated Hp /Hp ratio (AUC=0.8449) and ELISA Index (AUC=0.8581) had better performance in distinguishing HCC from controls, which indicated that fucosylated Hp ratios could improve the diagnosis and prediction of HCC even with a low level of alpha-fetoprotein (AFP). Additionally, the combination analysis of AFP and fucosylated Hp ratios increased the AUC value for HCC diagnosis.