RETROVIRAL DNA H-STRUCTURES - DISPLACEMENT-ASSIMILATION MODEL OF RECOMBINATION
RETROVIRAL DNA H-STRUCTURES - DISPLACEMENT-ASSIMILATION MODEL OF RECOMBINATION
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DOI：
10.1016/0092-8674(82)90011-3
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
SKALKA, AM
中科院分区：
文献类型：
--
作者：
JUNGHANS, RP;BOONE, LR;SKALKA, AM
在已知的RNA和DNA病毒中，禽逆转录病毒的基因重组频率极高，是独一无二的。这些高频率显然可以通过以下事实来解释：这种二倍体病毒密切相关的RNA基因组可以同时进行逆转录，并且链位移是逆转录反应的基本特性。EM高频观察到的新结构的特性提出了一个描述这一过程的特定模型：二聚体排列的DNA双链体通过单链DNA桥连接在同源区域。这些结构被认为是重组的中间产物，因为它们在体外的产生阻止了感染期间通常通过细胞装置进行的后续分解步骤。该模型产生了几个假设，这些假设的探索应该有助于检验其准确性。
The avian retroviruses are unique among known RNA and DNA viruses in their extremely high frequencies of genetic recombination. These high frequencies apparently can be explained by the facts that the closely associated RNA genomes of this diploid virus can be reverse-transcribed concurrently and that strand displacement is a fundamental property of the reverse transcription reaction. A specific model to describe this process was suggested by the properties of novel structures observed with high frequency by EM: DNA duplexes in dimer arrangement that are linked at homologous regions by single-stranded DNA bridges. These structures are presumed to be intermediates of recombination, trapped because their generation in vitro prevents the subsequent resolution steps that would normally take place via the cellular apparatus during infection. The model generates several hypotheses whose exploration should help to test its accuracy.