[Implantation: the first maternal-embryo crosstalk].
[Implantation: the first maternal-embryo crosstalk].
复制标题
DOI：
--
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
S. Perrier d’Hauterive
中科院分区：
文献类型：
--
作者：
S. Perrier d’Hauterive
尽管辅助生殖技术取得了进步，但缺乏对植入的控制仍然是获得成功怀孕的主要障碍。确定容受性子宫内膜的特征是至关重要的。胚胎着床是一个复杂的过程，它涉及到胚泡与母体子宫内膜的粘连，最后是子宫内膜的侵袭。虽然胚胎植入可以发生在任何人体组织中，但子宫内膜是唯一不能进行胚胎植入的组织，除非在被称为“植入窗口”的限制性时期内。在这段时间内，子宫内膜对胚胎的接受度很高。母体-胚胎串扰更受植入窗的青睐。植入窗的分子基础尚不明确，本研究探讨了hCG、TGFbeta1、IGF1和IGF2在体外培养的人子宫内膜上皮细胞分泌白血病抑制因子(LIF)和白介素6(IL-6)中的作用。从28例育龄妇女的子宫内膜活检中获得子宫内膜上皮细胞。在原代培养的人子宫内膜上皮细胞中加入人绒毛膜促性腺激素和转化生长因子β1，对LIF的分泌有促进作用，对IL-6的分泌有抑制作用。所有这些发现表明hCG和TGFbeta1在人类胚胎着床中起作用。
Despite progress in assisted reproduction technologies, the lack of control of implantation remains a major obstacle to obtain successful pregnancies. It is of prime importance to determine the characteristic features of a receptive endometrium. Embryo implantation is a complex event involving apposition followed by the adhesion of the blastocyst to the maternal endometrium, and finally the invasion of this endometrium. Though implantation could occur in any human tissue, the endometrium is the only tissue where embryo implantation cannot occur except during a restricted period called the "implantation window". During this window, the endometrium is highly receptive to the embryo. Maternal-embryo crosstalk is favored by the implantation window. The molecular basis of the implantation window remains to be defined In the present study, we investigated the role of hCG, TGFbeta1, IGF1 and IGF2 in the secretion of leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) by human epithelial endometrial cells in culture. Epithelial endometrial cells were obtained from endometrial biopsy in 28 fertile women. HCG and TGFbeta1 added to primary cultures of human epithelial endometrial cells showed a stimulatory effect on LIF secretion and a reducing effect on IL-6 secretion. All these findings suggest a role for hCG and TGFbeta1 in human embryo implantation.