Thin-film IrOx pH microelectrode for microfluidic-based microsystems
Thin-film IrOx pH microelectrode for microfluidic-based microsystems
复制标题
DOI：
10.1016/j.bios.2004.09.021
复制
发表时间：
2005-08-15
影响因子：
12.6
通讯作者：
Baudenbacher, FJ
中科院分区：
文献类型：
--
作者：
Ges, IA;Ivanov, BL;Baudenbacher, FJ
微传感器是监测细胞培养体积中细胞代谢的重要工具。本研究描述了片上薄膜铱、氧化物 pH 微传感器的制造和表征，其尺寸为 20 μm x 20 μm 和 20 μm x 40 μm，适合纳入 nl 体积。通过恒电流模式电化学沉积在铂微电极上形成 IrOx 薄膜。阳极生长的氧化铱薄膜表现出接近超能斯特响应，22℃时斜率为-77.6 +/- 2 mV/pH，并且在pH 4-11范围内呈线性响应。新沉积的电极显示响应时间低至 6 秒。长期研究表明基线漂移为 2-3 mv/月，可以通过校准轻松补偿。这项工作首次证明了使用平面 IrOx pH 微电极在微流体控制下测量 25 nl 芯片上细胞培养体积中 CHO 和成纤维细胞的酸化率。 (c) 2004 Elsevier B.V. 保留所有权利。
Microsensors are valuable tools to monitor cell metabolism in cell culture volumes. The present research describes the fabrication and characterization of on-chip thin-film iridium, oxide pH microsensors with dimensions of 20 mu m x 20 mu m and 20 mu m x 40 mu m suitable to be incorporated into nl volumes. IrOx thin films were formed on platinum microelectrodes by electrochemical deposition in galvanostatic mode. Anodically grown iridium oxide films showed a near super-Nernstian response with a slope of -77.6 +/- 2 mV/pH at 22 degrees C, and linear responses within the pH range of 4-11. Freshly deposited electrodes showed response times as low as 6 s. Long-term studies showed a baseline drift of 2-3 mv/month, which could easily be compensated by calibration. This work demonstrated for the first time the use of planar IrOx pH microelectrodes to measure the acidification rate of CHO and fibroblast cells in an on chip cell culture volume of 25 nl with microfluidic control. (c) 2004 Elsevier B.V. All rights reserved.