Medium optimization for the production of cyclic adenosine 3′,5′-monophosphate by Microbacterium sp no. 205 using response surface methodology
Medium optimization for the production of cyclic adenosine 3′,5′-monophosphate by Microbacterium sp no. 205 using response surface methodology
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DOI：
10.1016/j.biortech.2008.07.062
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发表时间：
2009-01-01
影响因子：
11.4
通讯作者：
Ying, Han-Jie
中科院分区：
文献类型：
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作者：
Chen, Xiao-Chun;Bai, Jian-Xin;Ying, Han-Jie
采用响应面法优化微杆菌属205号菌株生产环腺苷酸（cAMP）的培养基成分。应用部分因子设计（2^(11 - 7)）评估培养基中不同成分的影响。发现磷酸氢二钾（K₂HPO₄）、硫酸镁（MgSO₄）和氟化钠（NaF）对cAMP的生产有显著影响。采用最陡爬坡法确定培养基成分的最优区域，随后利用中心复合设计和响应面法对这三个因素的浓度进行优化。确定了实现cAMP最优生产的最佳培养基成分（克/升）：磷酸氢二钾，12.78；硫酸镁，3.53；氟化钠，0.18。相应的cAMP浓度为8.50克/升，与使用原始培养基相比提高了约1.8倍。还进行了验证实验以证明所获模型的充分性和准确性。在5升发酵罐中的cAMP发酵量达到9.87克/升。（C）2008爱思唯尔有限公司。保留所有权利。
Response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3',5'-monophosphate (cAMP) with Microbacterium sp. no. 205. A fractional factorial design (2(11 - 7)) was applied to evaluate the effects of different components in the medium. K2HPO4, MgSO4 and NaF were found to significantly influence on the cAMP production. The steepest ascent method was used to access the optimal region of the medium composition, The concentrations of the three factors were optimized subsequently using central composite design and response surface methodology. The optimal medium composition to achieve the optimal cAMP production was determined (g/L): K2HPO4, 12.78; MgSO4, 3.53 and NaF, 0.18. The corresponding cAMP concentration was 8.50 g/L, which was about 1.8-fold increase compared with that using the original medium. Validation experiments were also carried out to prove the adequacy and the accuracy of the model obtained. The cAMP fermentation in 5 L fermenter reached 9.87 g/L. (C) 2008 Elsevier Ltd. All rights reserved.