REQUIREMENT FOR HOST TRANSCRIPTION IN THE REPLICATION OF SINDBIS VIRUS
REQUIREMENT FOR HOST TRANSCRIPTION IN THE REPLICATION OF SINDBIS VIRUS
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DOI：
10.1128/jvi.45.1.200-205.1983
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
JOHNSTON, RE
中科院分区：
文献类型：
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作者：
BARIC, RS;CARLIN, LJ;JOHNSTON, RE
在用放线菌素D(AMD)或α-氨基丁酸(α-A)处理的细胞中，检测宿主细胞参与辛德比斯病毒(SB)的复制。在感染前用这些宿主转录抑制物处理使细胞支持SB生长的能力降低了1-2个数量级，而对水泡性口炎病毒的复制几乎没有影响。在野生型中国仓鼠卵巢(CHO)细胞中，SB复制对α-A敏感，而在CHOama-1细胞中，SB复制对α-A敏感，而在CHOama-1细胞中，SB的复制对α-A敏感。SBamr不仅在AMD处理的细胞中正常生长，而且在α-A处理的细胞中也正常生长。显然，复制SB需要细胞mRNA(可能还有蛋白质)的合成，用任何一种药物预先治疗都会影响SB复制的同一方面，SB基因组中的突变使病毒能够克服宿主转录抑制物的影响。
Host cell involvement in Sindbis virus (SB) replication was examined in cells which had been treated with either actinomycin D (AMD) or .alpha.-amanitin (.alpha.-A). Treatment with these inhibitors of host transcription before infection reduced the ability of cells to support SB growth by 1-2 orders of magnitude, while having little or no effect on the replication of vesicular stomatitis virus. SB replication was sensitive to .alpha.-A in wild-type Chinese hamster ovary (CHO) cells but was resistant to .alpha.-A in CHOama-1 cells, a line which contains an .alpha.-A-resistant RNA polymerase II. A mutant of SB, SBamr, was isolated by mutagenesis followed by selection in cells which had been treated with AMD. SBamr grew normally not only in cells treated with AMD but also in .alpha.-A-treated cells. Evidently, the synthesis of cellular mRNA (and presumably protein) is required for replication of SB, prior treatment with either drugs affects the same aspect of SB replication and mutations in the SB genome allow the virus to overcome the effect of inhibitors of host transcription.