FLUORESCENCE CORRELATION SPECTROSCOPY .3. UNIFORM TRANSLATION AND LAMINAR-FLOW
FLUORESCENCE CORRELATION SPECTROSCOPY .3. UNIFORM TRANSLATION AND LAMINAR-FLOW
复制标题
DOI：
10.1002/bip.1978.360170208
复制
发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
ELSON, EL
中科院分区：
文献类型：
--
作者：
MAGDE, D;WEBB, WW;ELSON, EL
荧光相关光谱扩展到在样品池中经历平移或层流的系统。包括理论和实验结果;均匀和不均匀的速度分布被认为是。浓度相关性分析提取微观速率参数的测量自发浓度波动，即使在平衡。荧光是监测这些波动的最灵敏的手段之一。流动或平移系统的分析提供了一种测量所选物质的数量浓度的方法，即，聚集体或聚合物，提供了一个非扰动的速度探针，有时允许一个规避光解降解，已被证明是非常有帮助的测试和校准装置的荧光相关测量和验证理论分析，并可能需要解释的结果获得的系统在运动中，即使该运动是不希望的或最初未被怀疑。结合Poiffille流和扩散的理论和实验结果包括在几何形状，这是最实际的利益。非扩散Poiffille流以及均匀流或有或无扩散的平移的简单得多的情况的理论表达式构成了明确显示的极限情况。
Fuorescence correlation spectroscopy is extended to systems that undergo translation or laminar flow in a sample cell. Theoretical and experimental results are included; uniform and non-uniform velocity profiles are considered. Concentration correlation analysis extracts microscopic rate parameters from measurements of the spontaneous concentration fluctuations, which occur even at equilibrium. Fluorescence is one of the most sensitive means of monitoring these fluctuations. Analysis of flowing or translating systems offers a method of measuring number concentrations of selected species, i.e., of aggregates or polymers, provides a nonperturbing velocity probe, sometimes allows one to circumvent photolytic degradation, has proved extremely helpful in testing and aligning apparatus for fluorescence correlation measurements and in verifying theoretical analyses and may be required for interpretation of results obtained on systems in motion, even though that motion is undesired or initially unsuspected. Theoretical and experimental results for combined Poiseuille flow and diffusion are included in the geometry which is of most practical interest. Theoretical expressions for the much simpler cases of nondiffusive Poiseuille flow as well as uniform flow or translation with or without diffusion constitute limiting cases which are displayed explicitly.