Requirements for CD1d Recognition by Human Invariant Vα24+ CD4−CD8− T Cells
Requirements for CD1d Recognition by Human Invariant Vα24+ CD4−CD8− T Cells
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发表时间：
1997-07
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通讯作者：
M. Exley;Jorge A. Garcia;S. Balk;S. Porcelli
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作者：
M. Exley;Jorge A. Garcia;S. Balk;S. Porcelli
人类CD_4−CD_8−T细胞亚群表达不变的Vα24-JαQ T细胞受体(TCR)-α链，主要与Vβ11配对。其中一系列Vα24 Vβ11克隆具有TcR-βCDR3多样性，并表达自然杀伤(NK)位点编码的C型凝集素NKR-P1a、CD94和CD69。然而，与NK细胞不同的是，它们不表达杀伤抑制受体CD16、CD56或CD57。所有不变的Vα24+克隆均识别类MHCCD16分子，并区分CD1d和其他密切相关的人类CD1蛋白，表明这种识别是由TcR介导的。识别不依赖于CD1d细胞质尾部的内体靶向基序。在抗CD3或CD1d的激活下，克隆产生Th1和Th2细胞因子。这些结果表明，人不变Vα2 4+CD4NK8CD8T细胞和同源小鼠−+T细胞群具有CD1d反应性，其功能与NK细胞不同。这种细胞群体和CD1d配体跨物种的保守性表明了一种重要的免疫功能。
A subset of human CD4−CD8− T cells that expresses an invariant Vα24-JαQ T cell receptor (TCR)-α chain, paired predominantly with Vβ11, has been identified. A series of these Vα24 Vβ11 clones were shown to have TCR-β CDR3 diversity and express the natural killer (NK) locus–encoded C-type lectins NKR-P1A, CD94, and CD69. However, in contrast to NK cells, they did not express killer inhibitory receptors, CD16, CD56, or CD57. All invariant Vα24+ clones recognized the MHC class I–like CD16 molecule and discriminated between CD1d and other closely related human CD1 proteins, indicating that recognition was TCR-mediated. Recognition was not dependent upon an endosomal targeting motif in the cytoplasmic tail of CD1d. Upon activation by anti-CD3 or CD1d, the clones produced both Th1 and Th2 cytokines. These results demonstrate that human invariant Vα24+ CD4−CD8− T cells, and presumably the homologous murine NK1+ T cell population, are CD1d reactive and functionally distinct from NK cells. The conservation of this cell population and of the CD1d ligand across species indicates an important immunological function.