Viral protein U counteracts a human host cell restriction that inhibits HIV-1 particle production
Viral protein U counteracts a human host cell restriction that inhibits HIV-1 particle production
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DOI：
10.1073/pnas.2433165100
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发表时间：
2003-12-09
影响因子：
11.1
通讯作者：
Spearman, P
中科院分区：
文献类型：
--
作者：
Varthakavi, V;Smith, RM;Spearman, P
人体细胞通过多种机制抵抗病毒感染。病毒必须克服宿主细胞的限制才能成功地复制其遗传物质。在这里，我们确定了宿主在粒子组装阶段对病毒复制的限制。病毒蛋白U （Vpu）是一种HIV-1辅助蛋白，在大多数人类细胞中增强颗粒组装和释放，但在猿类细胞中没有。通过使用人类-猿猴细胞异核体，我们发现在人类细胞中对组装的抑制是显性的。Vpu克服了在人类细胞和人猿异核体中组装的障碍。HIV-1 vpu基因可能已经进化为抵消存在于人类细胞中的组装限制。
Human cells resist viral infections by a variety of mechanisms. Viruses must overcome host cell restrictions to successfully reproduce their genetic material. Here, we identify a host restriction to viral replication that acts at the stage of particle assembly. Viral protein U (Vpu) is an HIV-1 accessory protein that enhances particle assembly and release in most human cells, but not in simian cells. By using human-simian cell heterokaryons, we show that the inhibition of assembly in human cells is dominant. Vpu overcomes the block to assembly in human cells and in human-simian heterokaryons. The HIV-1 vpu gene may have evolved to counteract an assembly restriction that is present in human cells.