Altered Venous Function in Hypertensive Rats
Altered Venous Function in Hypertensive Rats
复制标题
高血压大鼠静脉功能的改变
DOI：
10.1161/01.res.38.5.412
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发表时间：
1976
影响因子：
20.1
通讯作者：
G. Simon
中科院分区：
文献类型：
--
作者：
G. Simon
在37 ℃下，用含葡聚糖（7 g/100 ml）的氧合Krebs-Ringer溶液通过主动脉灌注大鼠上身或下身的血管床。每隔10分钟停止灌注，并在快速输注（15.3-90.0 ml/min）Krebs-Ringer溶液至下腔静脉期间记录颈静脉或股静脉中的压力升高。本实验选用9-11月龄新西兰系雄性遗传性高血压大鼠（GHR）6只，5-6月龄新西兰系雌性GHR 7只，4月龄冈本系雄性自发性高血压大鼠（SHR）8只，夹闭后30天的两肾Goldblatt高血压大鼠（2-KGH）5只。（5）7只雄性2-KGH大鼠，夹闭后65天;（6）8只单肾Goldblatt高血压（1-KGH）雄性大鼠，夹闭后40天;（7）适当品系的体重和性别匹配的正常血压对照大鼠;和（8）体重和性别匹配的双肾和单肾假夹正常血压大鼠。初步研究表明，快速输注到静脉循环中填充静脉，但没有液体进入循环的动脉侧。与对照组相比，GHR（雌雄）、SHR、1-KGH和2-KGH大鼠在夹闭后65天（而非30天）的静脉压力-容积曲线均向压力轴方向移动（P < 0.05）。静脉压力-容积曲线的移动持续后，通过杀死大鼠或通过硝普钠（0.1毫克/毫升灌注液）的管理，或两者的血管收缩张力降低。研究结果表明，静脉容量降低高血压大鼠。
The vascular beds of the upper or lower body of rats were perfused through the aorta with oxygenated Krebs-Ringer solution containing dextran (7 g/100 ml), at 37°C. Perfusion was stopped every 10 minutes, and the pressure rise in the jugular or the femoral vein was recorded during rapid infusion (15.3–90.0 ml/min) of Krebs-Ringer solution into the inferior vena cava. The following groups of rats were studied: (1) six male genetically hypertensive rats (GHR), 9–11 months old, New Zealand strain; (2) seven female GHR, 5-6 months old, New Zealand strain; (3) eight male spontaneously hypertensive rats (SHR), 4 months old, Okamoto strain; (4) five male rats with two-kidney Goldblatt hypertension (2-KGH), 30 days postclipping; (5) seven male 2-KGH rats, 65 days postclipping; (6) eight male rats with one-kidney Goldblatt hypertension (1-KGH), 40 days postclipping; (7) weight- and sex-matched normotensive control rats of the appropriate strain; and (8) weight- and sex-matched two-kidney and one-kidney, sham-clipped normotensive rats. Preliminary studies showed that rapid infusion into the venous circulation fills the veins, but there is no entry of fluid into the arterial side of the circulation. Compared to controls, the venous pressure-volume curves of GHR (male and female), SHR, 1-KGH rats and 2-KGH rats, 65 (but not 30) days postclipping, were shifted toward the pressure axis (P < 0.05). The shift of the venous pressure-volume curves persisted following the reduction of vasoconstrictor tone by killing the rats or by the administration of sodium nitroprusside (0.1 mg/ml perfusate), or by both. The findings suggest decreased venous capacity in hypertensive rats.