Increased calcium permeability of cold-stored erythrocytes.
Increased calcium permeability of cold-stored erythrocytes.
复制标题
冷藏红细胞的钙渗透性增加。
作者：
J. Wiley;K. E. McCulloch;D. S. Bowden
研究了在 4 摄氏度下储存在各种抗凝介质中的血液中的红细胞的钙、钠和镁渗透性，并与新鲜红细胞的渗透性进行了比较。在经过预处理以消除 Ca2+ 泵送的细胞中，在 37 摄氏度下测量了 CA2+ 的被动流入，并且冷藏红细胞的 CA2+ 被动流入量比新鲜细胞高出五倍。在 ACD、CPD、CPD-腺嘌呤或肝素抗凝剂中，Ca2+ 渗漏在第 2 天后逐渐发展，并在冷藏第 7 天达到最大值。 ACD中冷藏红细胞的总钙含量与新鲜红细胞没有显着差异。然而，当冷藏的红细胞在含有 1.5 mM 离子钙和底物的培养基中于 37°C 下重新孵育以再生 ATP 时，发生的 Ca2+ 净增益在存储时比新鲜红细胞更大。血液在任何抗凝剂中冷藏长达 6 周都不会改变钠或镁的渗透性。还测量了红细胞 ATP，并在 ACD 或 CPD 冷藏期间稳定下降，但 Ca2+ 渗透性的增加先于红细胞 ATP 的任何显着变化，很可能选择性钙泄漏的发生与冷藏时发生的 ATP 浓度下降无关。
The calcium, sodium, and magnesium permeability of erythrocytes from blood stored at 4 degrees C in various anticoagulant media has been studied and compared to that of fresh erythrocytes. Passive influx of CA2+ was measured at 37 degrees C in cells pretreated to abolish Ca2+ pumping and was up to fivefold greater for cold-stored erythrocytes than for fresh cells. The Ca2+ leakiness developed gradually after day 2 and reached a maximum by day 7 of cold storage in ACD, CPD, CPD-adenine, or heparin anticoagulants. The total calcium content of cold-stored erythrocytes in ACD was not significantly different from that of fresh erythrocytes. However, when cold-stored erythrocytes were reincubated at 37 degrees C in media containing 1.5 mM ionized calcium and substrates to regenerate ATP, a net gain of Ca2+ occurred that was greater for stored than for fresh erythrocytes. Cold storage of blood for up to 6 wk in any anticoagulant did not alter either sodium or magnesium permeability. Red cell ATP was also measured and fell steadily during cold storage in ACD or CPD, but more increase in Ca2+ permeability preceded any significant change in red cell ATP, it is likely that a selective calcium leak develops independently of the fall in ATP concentration that occurs on cold storage.