Redox Linked Flavin Sites in Extracellular Decaheme Proteins Involved in Microbe-Mineral Electron Transfer.
Redox Linked Flavin Sites in Extracellular Decaheme Proteins Involved in Microbe-Mineral Electron Transfer.
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DOI：
10.1038/srep11677
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发表时间：
2015-07-01
影响因子：
4.6
通讯作者：
Clarke TA
中科院分区：
文献类型：
--
作者：
Edwards MJ;White GF;Norman M;Tome-Fernandez A;Ainsworth E;Shi L;Fredrickson JK;Zachara JM;Butt JN;Richardson DJ;Clarke TA
在许多地下环境中，胞外微生物-矿物电子传递是有机碳氧化的主要驱动力。希文氏菌属的细胞外多血红素细胞色素在这一过程中发挥了重要作用，但在细胞色素和受体之间的界面处的电子交换机制是广泛争论的。十血红素MtrC的1.8 X射线晶体结构揭示了高度保守的CX 8 C二硫化物，当其取代AX 8A时，严重损害了S.在有氧条件下生长。还原裂解的二硫化物的存在下，黄素单胞苷（FMN）导致在可逆的形成一个稳定的flavocytochrome。用其他十血红素细胞色素OmcA、MtrF和UndA也观察到类似的结果。这些数据表明，这些decaheme细胞色素可以过渡之间的高反应性flavocytochromes或反应性较低的细胞色素，这种过渡是由一个氧化还原活性二硫化物，响应氧气的存在下控制。
Extracellular microbe-mineral electron transfer is a major driving force for the oxidation of organic carbon in many subsurface environments. Extracellular multi-heme cytochromes of the Shewenella genus play a major role in this process but the mechanism of electron exchange at the interface between cytochrome and acceptor is widely debated. The 1.8 Å x-ray crystal structure of the decaheme MtrC revealed a highly conserved CX8C disulfide that, when substituted for AX8A, severely compromised the ability of S. oneidensis to grow under aerobic conditions. Reductive cleavage of the disulfide in the presence of flavin mononucleotide (FMN) resulted in the reversible formation of a stable flavocytochrome. Similar results were also observed with other decaheme cytochromes, OmcA, MtrF and UndA. The data suggest that these decaheme cytochromes can transition between highly reactive flavocytochromes or less reactive cytochromes, and that this transition is controlled by a redox active disulfide that responds to the presence of oxygen.
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影响因子：
3.7
作者：
Mitchell, A. C.;Peterson, L.;Geesey, G. G.
通讯作者：
Geesey, G. G.
DOI：
10.1107/s090744499900846x
发表时间：
1999-10-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Leslie, AGW
通讯作者：
Leslie, AGW
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
DOI：
10.1107/s0907444911001314
发表时间：
2011-04
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Murshudov GN;Skubák P;Lebedev AA;Pannu NS;Steiner RA;Nicholls RA;Winn MD;Long F;Vagin AA
通讯作者：
Vagin AA
DOI：
10.1107/s0907444994003112
发表时间：
1994-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
BAILEY, S
通讯作者：
BAILEY, S