An appraisal of tests for native DNA antibodies in connective tissue diseases. Clinical usefulness of Crithidia luciliae assay.
An appraisal of tests for native DNA antibodies in connective tissue diseases. Clinical usefulness of Crithidia luciliae assay.
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结缔组织疾病中天然 DNA 抗体测试的评估。
DOI：
10.7326/0003-4819-89-2-186
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发表时间：
1978
影响因子：
39.2
通讯作者：
M. Ziff
中科院分区：
文献类型：
--
作者：
A. Chubick;R. Sontheimer;J. Gilliam;M. Ziff
对天然DNA的抗体通过五种技术进行检测：光亮甲虫免疫荧光(CL-IF)试验；使用未经处理的人KB DNA、经核酸内切酶处理的KB DNA或合成多核苷酸(PolyDAT)的滤过性放射免疫分析；以及使用KB DNA的Farr免疫沉淀试验。CL-IF分析的特异性和敏感性与使用KB DNA的滤过式放射免疫分析方法相似。CL-IF试验显示，在活动性疾病和严重肾脏损害的患者中，阳性试验的频率增加。对肾损害严重的患者，用该程序检测血清中高滴度的nDNA抗体。CL-IF检测的一个独特优势是它能够识别补体结合nDNA抗体。这种抗体的存在与高抗体滴度和严重肾脏疾病的存在相关。CL-IF法是一种简便、实用的检测抗-nDNA的方法。
Antibody to native DNA was measured by five techniques: the Crithidia luciliae immunofluorescence (CL-IF) test; filter radioimmunoassays using either untreated human KB DNA, endonuclease-treated KB DNA, or a synthetic polynucleotide (poly dAT); and the Farr immunoprecipitation assay using KB DNA. The specificity and sensitivity of the CL-IF assay was similar to that of the filter radioimmunoassay procedures using KB DNA. The CL-IF test showed an increased frequency of positive tests in patients with active disease and severe renal involvement. In patients with severe renal involvement, high titers of serum nDNA antibodies were measured by this procedure. A unique advantage of the CL-IF test was its ability to identify complement-fixing nDNA antibody. The presence of such antibody was correlated with high antibody titer and the presence of severe renal disease. The CL-IF assay is a simple and useful procedure for measurement of anti-nDNA.