Dopaminergic projections to the medial preoptic area of postpartum rats.
Dopaminergic projections to the medial preoptic area of postpartum rats.
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DOI：
10.1016/j.neuroscience.2009.01.060
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发表时间：
2009-04-10
期刊：
影响因子：
3.3
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中科院分区：
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多巴胺受体在啮齿动物内侧视前区（mPOA）的活性对母性行为的表现以及许多其他生理和行为功能至关重要。然而，多巴胺能输入到mPOA的来源尚未通过神经解剖追踪确定。为了实现这一目标，将逆行示踪剂氟金离子电泳应用于产后实验室大鼠的mPOA，并对氟金和酪氨酸羟化酶进行双标记免疫细胞化学，以鉴定前脑和中脑投射到mPOA的多巴胺能细胞。结果表明，多巴胺能细胞投射到mPOA的数量适中（每半球约90个细胞），并且这些细胞的分布出乎意料地广泛。即便如此，超过一半的双标记细胞被发现在A10多巴胺组的延伸区域（特别是下丘脑腹侧侧后核和邻近的内侧乳上核），或腹侧被盖区的A10细胞中。下丘脑吻侧及周围区域也含有大量双标记细胞，其中mPOA内（包括AVPV和PVpo）的双标记细胞数量最多。值得注意的是，双标记细胞在incerta （A13）中很少见，A13是一个先前被认为向mPOA提供多巴胺能输入的部位。这项研究是第一个使用解剖追踪来详细描述实验室大鼠多巴胺能投射到mPOA的研究，并表明这种投射的大部分起源比以前认为的更晚。
Dopamine receptor activity in the rodent medial preoptic area (mPOA) is crucial for the display of maternal behaviors, as well as numerous other physiological and behavioral functions. However, the origin of dopaminergic input to the mPOA has not been identified through neuroanatomical tracing. To accomplish this, the retrograde tracer Fluorogold was iontophoretically applied to the mPOA of postpartum laboratory rats, and dual-label immunocytochemistry for Fluorogold and tyrosine hydroxylase later performed to identify dopaminergic cells of the forebrain and midbrain projecting to the mPOA. Results indicate that the number of dopaminergic cells projecting to the mPOA is moderate (~90 cells to one hemisphere), and that these cells have an unexpectedly wide distribution. Even so, more than half of the dual-labeled cells were found in what has been considered extensions of the A10 dopamine group (particularly the ventrocaudal posterior hypothalamus and adjacent medial supramammillary nucleus), or in the A10 cells of the ventral tegmental area. The rostral hypothalamus and surrounding region also contained numerous dual-labeled cells, with the greatest number found within the mPOA itself (including in the AVPV and PVpo). Notably, dual-labeled cells were rare in the zona incerta (A13), a site previously suggested to provide dopaminergic input to the mPOA. This study is the first to use anatomical tracing to detail the dopaminergic projections to the mPOA in the laboratory rat, and indicates that much of this projection originates more caudally than previously suggested.