Requirement for memory B-cell activation in protection from heterologous influenza virus reinfection
Requirement for memory B-cell activation in protection from heterologous influenza virus reinfection
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DOI：
10.1093/intimm/dxz049
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发表时间：
2019-12-01
影响因子：
4.4
通讯作者：
Kurosaki, Tomohiro
中科院分区：
文献类型：
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作者：
Leach, Sarah;Shinnakasu, Ryo;Kurosaki, Tomohiro
虽然记忆B细胞和长寿浆细胞这两个记忆区室被认为有助于记忆回忆反应的成功建立，但每个细胞区室的独特作用仍不清楚。在此，通过追踪小鼠中的流感抗血凝素（HA）特异性抗体，我们证明了由长寿浆细胞分泌的预先存在的抗体对于保护免受相同流感病毒的再次感染是必不可少的，而保护免受抗原性不同的流感病毒的二次感染需要记忆B细胞活化。这些激活的记忆B细胞对保守的HA干细胞区有很大的特异性，并产生足够水平的抗体以防止异源再感染。鉴于来自记忆B细胞的抗干细胞浆母细胞比来自幼稚B细胞的那些具有更高的亲和力，我们的研究结果表明，抗干细胞记忆B细胞在原发性流感感染期间的成熟及其随后的激活是防止突变病毒再感染所必需的。
While two memory compartments, memory B cells and long-lived plasma cells, are thought to contribute to the successful establishment of memory recall responses, the unique roles of each cellular compartment are still unclear. Herein, by tracing influenza anti-hemagglutinin (HA)-specific antibodies in mice, we demonstrate that pre-existing antibodies secreted by long-lived plasma cells are essential for protection from reinfection with the same influenza virus, whereas protection from secondary infection with an antigenically distinct influenza virus requires memory B-cell activation. These activated memory B cells were largely specific for the conserved HA stem region, and generated sufficient levels of antibodies for protection from heterologous reinfection. Given that the anti-stem plasmablasts derived from the memory B cells were higher affinity than those from naive B cells, our results suggest that maturation of anti-stem memory B cells during primary influenza infection and their subsequent activation are required for protection from reinfection by mutant viruses.