COPPER STATUS AND FUNCTION OF NEUTROPHILS ARE REVERSIBLY DEPRESSED IN MARGINALLY AND SEVERELY COPPER-DEFICIENT RATS
COPPER STATUS AND FUNCTION OF NEUTROPHILS ARE REVERSIBLY DEPRESSED IN MARGINALLY AND SEVERELY COPPER-DEFICIENT RATS
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DOI：
10.1093/jn/120.12.1700
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发表时间：
1990-12-01
影响因子：
4.2
通讯作者：
FAILLA, ML
中科院分区：
文献类型：
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作者：
BABU, U;FAILLA, ML
给断奶雄性大鼠配对饲喂含铜适量(7 mg/kg饲料；+铜)和低铜(0.7 mg/kg饲料；-铜)的饲料，持续5周。中性粒细胞铜浓度、铜锌超氧化物歧化酶活性、对酵母多糖和佛波酯的反应产生超氧阴离子(O2-)、中性粒细胞杀灭念珠菌活性均显著降低。此外，将白色念珠菌注射到缺铜大鼠的腹膜腔内，其存活率高于对照组。中性粒细胞的吞噬活性与细胞铜状态无关。为了确定中性粒细胞功能受损是否也是边缘铜缺乏的特征，给断奶大鼠饲喂含铜0.6、1.1、2.0、2.7或6.7 mg/kg(对照组)的饲料5wk。中性粒细胞的铜锌超氧化物歧化酶活性、佛波酯诱导的O2-产生和杀灭念珠菌活性均显著低于对照组。相反，只有当饲料中铜含量低于或等于2.0 mg/kg时，组织铜含量和红细胞铜、锌-超氧化物歧化酶活性才会降低。中性粒细胞的超氧化物歧化酶活性、呼吸爆发和杀菌活性在开始饮食治疗后1wk即明显降低。给缺铜大鼠喂饲+铜饲料1wk后，中性粒细胞的铜状态和功能恢复到对照水平。这些结果表明，轻度和严重的铜缺乏可使中性粒细胞的功能发生可逆的损害，提示这些细胞的铜状态和功能可能为铜状态的评估提供一个敏感的指标。
Weaned male rats were pair-fed diets containing either adequate (7 mg/kg diet; +Cu) or deficient (0.7 mg/kg diet; -Cu) levels of copper for 5 wk. Cellular Cu concentration, activity of Cu, Zn superoxide dismutase (Cu, Zn-SOD), generation of superoxide anion (O2-) in response to opsonized zymosan and phorbol myristate acetate, and candidacidal activity in elicited neutrophils were all significantly decreased by dietary Cu deficiency. Furthermore, survivability of Candida albicans injected into the peritoneal cavity of Cu-deficient rats was greater than in controls. Phagocytic activity of neutrophils was independent of cellular Cu status. To determine whether the impaired function of neutrophils was also characteristic of marginal Cu deficiency, weaned rats were fed diets containing either 0.6, 1.1, 2.0, 2.7 or 6.7 (control) mg Cu/kg for 5 wk. The activity of Cu,Zn-SOD, phorbol myristate acetate-induced production of O2-, and candidacidal activity were significantly lower in neutrophils from rats fed diets with less-than-or-equal-to-2.7 mg Cu/kg compared to control cells. In contrast, decreased tissue Cu and the activity of erythrocyte Cu,Zn-SOD were observed only when dietary Cu was less-than-or-equal-to-2.0 mg/kg. Reduced SOD activity, respiratory burst and candidacidal activity of neutrophils were evident as soon as 1 wk after initiating the dietary treatment. Feeding +Cu diet to Cu-deficient rats for 1 wk restored Cu status and function of neutrophils to control levels. These results show that neutrophil function is reversibly impaired by marginal and severe Cu deficiency and suggest that the Cu status and function of these cells may provide a sensitive indicator for assessment of Cu status.