The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus
The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus
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DOI：
10.1016/s0014-5793(02)02371-2
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发表时间：
2002-03-13
期刊：
影响因子：
3.5
通讯作者：
Watanabe, M
中科院分区：
文献类型：
--
作者：
Endo, M;Matsubara, H;Watanabe, M
为了了解生殖器官发育的内在分子机制，使用cDNA微阵列，从花蕾cDNA克隆制造，分离基因，这是在花药和雌蕊的发育过程中，在莲花的花药和雌蕊的特异性表达。聚类分析的微阵列数据揭示了21和111个独立的cDNA组，这是专门在未成熟和成熟花药，分别。进行RT-PCR以提供对我们方法的准确性和再现性的直接评估。我们的研究结果证实，cDNA微阵列技术是一种有效的工具，用于识别新的生殖器官特异性基因。(C)2002年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
To understand the molecular mechanisms intrinsic to reproductive organ development a cDNA microarray, fabricated from flower bud cDNA clones, was used to isolate genes, which are specifically expressed during the development of the anther and pistil in Lotus japonicus. Cluster analysis of the microarray data revealed 21 and 111 independent cDNA groups, which were specifically expressed in immature and mature anthers, respectively. RT-PCR was performed to provide a direct assessment of the accuracy and reproducibility of our approach. Confirmation of our results suggests that cDNA microarray technology is an effective tool for identification of novel reproductive organ-specific genes. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.