Ontogeny of the follicular dendritic cell phenotype and function in the postnatal murine spleen
Ontogeny of the follicular dendritic cell phenotype and function in the postnatal murine spleen
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DOI：
10.1006/cimm.2001.1874
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发表时间：
2001-11-25
影响因子：
4.3
通讯作者：
Szakal, AK
中科院分区：
文献类型：
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作者：
Balogh, P;Aydar, Y;Szakal, AK
滤泡树突状细胞（FDC）代表了一种独特的抗原捕获细胞群，仅限于次级淋巴组织内的滤泡。 FDC 似乎参与淋巴组织个体发育过程中初级滤泡的形成。我们试图使用针对 FDC 的单克隆抗体 (mAb) 的免疫组织化学以及体内免疫复合物结合和保留来确定表达仅限于 FDC 的各种分化抗原的新生小鼠脾脏中细胞的动力学和组织分布。显示的最早的 FDC 特异性标记物是 FDC-M1 mAb 识别的抗原决定簇，在卵泡形成前的第 3 天，位于发育中白髓周围部分周围的细胞上可检测到该标记物。在第一周结束时观察到 CD21/35（补体受体 2 型和 1 型，CR1.2）的出现，揭示了 B 细胞丰富区域的局灶模式。另外，当时在动脉周围区域的非滤泡部分也存在一些FDC-M1阳性细胞。将抗辣根过氧化物酶抗体和可溶性抗原 HRP 注射到 7 天大的新生小鼠中，即使在不存在 Fcgamma 受体的情况下，也会导致免疫复合物被捕获并保留在 FDC 上。另一种 FDC 特异性标记 FDC-M2 的出现是在出生后第二周观察到的，并且仅限于与 CR1.2 细胞位于同一区域的细胞。 Fcgamma 受体 II 型在产后第二周后出现在 FDC 上。上述表型成熟的顺序也可以在致死辐射后第 3 天的新生儿中观察到。这表明不仅成熟的 FDC，而且它们的前体都具有高度的抗辐射性，并且它们的表型成熟遵循仅需要少量成熟 B 细胞的程序化路径。 (C) 2001 年爱思唯尔科学（美国）。
Follicular dendritic cells (FDCs) represent a unique cell population of antigen trapping cells restricted to follicles within the secondary lymphoid tissues. FDCs appear to be involved in the formation of primary follicles during the ontogeny of lymphoid tissue. We sought to determine the kinetics and tissue distribution of cells in the spleen of newborn mice expressing various differentiation antigens restricted to FDCs using immunohistochemistry with monoclonal antibodies (mAb) against FDCs and in vivo immune complex binding and retention. The earliest FDC-specific marker displayed was the antigenic determinant recognized by the FDC-M1 mAb, which was detectable by Day 3 prior to follicle formation on cells located around the peripheral part of the developing white pulp. The appearance of CD21/35 (complement receptor Type 2 and 1, CR1.2) was observed at the end of the first week, revealing a focal pattern in B-cell-rich areas. In addition, at that time there were some FDC-M1-positive cells in the nonfollicular part of the periarteriolar region. The administration of anti-horseradish peroxidase antibody followed by soluble antigen HRP into 7-day-old newborn mice resulted in the trapping and retention of immune complexes onto FDCs even in the absence of Fcgamma receptors. The appearance of another FDC-specific marker, FDC-M2, was observed during the second week after birth and was restricted on the cells located in the same area as CR1.2 cells. The Fcgamma receptor Type II appeared on FDCs after the second postnatal week. The above sequence of phenotypic maturation could also be observed in newborns after lethal irradiation at Day 3. This indicates that not only mature FDCs but also their precursors are highly radioresistant, and their phenotypic maturation follows a programmed path that requires only a small number of mature B cells. (C) 2001 Elsevier Science (USA).