Osteoarthritis prevention and meniscus regeneration induced by transplantation of mesenchymal stem cell sheet in a rat meniscal defect model.
Osteoarthritis prevention and meniscus regeneration induced by transplantation of mesenchymal stem cell sheet in a rat meniscal defect model.
复制标题
间充质干细胞片移植在大鼠半月板缺损模型中预防骨关节炎和诱导半月板再生
DOI：
10.3892/etm.2016.3325
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发表时间：
2016-07
影响因子：
2.7
通讯作者：
Feng G
中科院分区：
文献类型：
--
作者：
Qi Y;Chen G;Feng G
间充质干细胞（MSCs）移植是半月板再生的潜在治疗方法。然而，当使用单细胞悬浮液注射时，通常会有大量细胞损失，只有一小部分细胞留在靶部位。这个问题可以通过使用MSC表来解决。在本研究中，我们研究了MSC薄片能否在大鼠半月板切除模型中有效地再生半月板。10只大鼠内侧半月板前半月板切除，骨髓间充质干细胞板治疗组移植骨髓间充质干细胞板，未治疗组为对照组。术后4周、8周分别对膝关节进行大体和组织学观察。组织学观察显示，半月板前部与天然组织相似，MSC薄片组显示典型的纤维软骨细胞被更丰富的细胞外基质包围。此外，观察到主要的富胶原基质桥接界面，新半月板与宿主半月板融合良好。两组患者胫骨平台及股骨髁均出现退行性改变。骨髓间充质干细胞片移植可有效缓解退行性改变。综上所述，骨髓间充质干细胞片移植可有效促进半月板再生，抑制膝关节骨关节炎的进展。
Transplantation of mesenchymal stem cells (MSCs) is a potential therapy for meniscus regeneration. However, when using single cell suspension injection, there is frequently a significant loss of cells, with only a small percentage of cells remaining at the target site. This issue may be solved with the use of MSC sheets. In the present study, we investigated whether the use of MSC sheets were able to regenerate the meniscus effectively in a rat meniscectomized model. The anterior half of the medial meniscus in 10 rats was excised and an MSC sheet was transplanted in the MSC sheet treatment group, while untreated rats served as the control. After 4 and 8 weeks, the knee joints were examined by gross and histological observation. Histological observation revealed that the anterior portion of meniscus was similar to the native tissue, showing typical fibrochondrocytes surrounded by richer extracellular matrix in the MSC sheet group. In addition, predominant collagen-rich matrix bridging the interface was observed and the neo-meniscus integrated well with its host meniscus. Furthermore, degenerative changes of tibial plateau and femoral condyle occurred in the two groups. MSC sheet transplantation alleviated the degenerative changes efficiently. In conclusion, transplantation of MSC sheets may efficiently promote meniscus regeneration, as well as inhibit the progression of osteoarthritis in knee joints.