IDENTIFICATION OF JUVENILE HORMONE-III IN THE HEMOLYMPH OF BLATTELLA-GERMANICA ADULT FEMALES BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY
IDENTIFICATION OF JUVENILE HORMONE-III IN THE HEMOLYMPH OF BLATTELLA-GERMANICA ADULT FEMALES BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY
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DOI：
10.1002/arch.940060306
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发表时间：
1987-11-01
影响因子：
2.2
通讯作者：
MESSEGUER, A
中科院分区：
文献类型：
--
作者：
CAMPS, F;CASAS, J;MESSEGUER, A
用气相色谱-质谱联用化学电离和选择离子监测的方法，从德国小蠊成虫血淋巴提取液中鉴定出保幼激素III。在此条件下，在该分析方法的灵敏度范围内未检测到保幼激素I和II。为了定量的目的，合成了保幼激素III的5，5-双氚类似物，并将其用作内标。一般来说，获得的保幼激素III效价与卵母细胞生长的数据以及沿着第一个性腺营养周期体外培养的鼻侧小体的激素效价相关。
Juvenile hormone III was identified in purified hemolymph extracts of adult females of the cockroach Blatella germanica by gas chromatography-mass spectrometry with chemical ionization and selected ion monitoring. Under these conditions, juvenile hormones I and II were not detected within the sensitivity ranges of this analytical method. For quantification purposes a 5,5-bisdeuterated analog of juvenile hormone III was synthesized and used as an internal standard. In general, juvenile hormone III titers obtained correlated with data on oocyte growth and with hormone titers found from in vitro corpora allata incubations along the first gonotrophic cycle.