Influence of cultivation media on genetic regulatory patterns in Borrelia burgdorferi.
Influence of cultivation media on genetic regulatory patterns in Borrelia burgdorferi.
复制标题
培养基对伯氏疏螺旋体遗传调控模式的影响。
DOI：
10.1128/iai.69.6.4159-4163.2001
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发表时间：
2001
影响因子：
3.1
通讯作者：
Norgard,MV
中科院分区：
文献类型：
--
作者：
Yang,X;Popova,TG;Goldberg,MS;Norgard,MV
Barbour-Stoenner-Kelly II (BSKII) 培养基和 BSKH 培养基均常规用于伯氏疏螺旋体的培养。然而，迄今为止还没有研究比较这两种介质如何影响 virulentB 中的基因表达模式。博格多弗里。在本研究中，我们发现一些B.通常受温度或 pH 调节的 burgdorferisttrain 297 基因（例如，ospA、mlp-7A、mlp-8、p22 和 lp6.6）在 B.伯氏疏螺旋体在 BSKH 培养基中培养；当螺旋体在传统 BSKII 培养基中培养时，情况并非如此。结果表明，对于 B.B. 基因表达研究，BSKH 培养基优于 BSKII 培养基。博格多弗里。
Barbour-Stoenner-Kelly II (BSKII) medium and BSKH medium both are routinely used for the cultivation ofBorrelia burgdorferi. However, heretofore there have been no studies to compare how these two media affect gene expression patterns in virulentB. burgdorferi. In the present study, we found that someB. burgdorferistrain 297 genes (e.g.,ospA,mlp-7A,mlp-8,p22, andlp6.6) that typically are regulated by temperature or pH displayed their predicted pattern of expression whenB. burgdorferiwas cultivated in BSKH medium; this was not true when spirochetes were cultivated in conventional BSKII medium. The results suggest that BSKH medium is superior to BSKII medium for gene expression studies withB. burgdorferi.