Protective effect of metallothionein against the toxicity of cadmium and other metals
Protective effect of metallothionein against the toxicity of cadmium and other metals
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DOI：
10.1016/s0300-483x(01)00375-4
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发表时间：
2001-06-21
期刊：
影响因子：
4.5
通讯作者：
Klaassen, CD
中科院分区：
文献类型：
--
作者：
Park, JD;Liu, YP;Klaassen, CD
金属硫蛋白（MT）分子量小。结合金属的富含半胱氨酸的蛋白质。MT对Cd毒性的保护作用已得到证实，但其对其他金属毒性的保护能力尚不清楚。本研究以野生型和MT- i和-II缺失小鼠（MT-null小鼠）为实验对象，研究MT是否不仅对Cd有保护作用，而且对Zn、Cu、Fe、Pb也有保护作用。汞和砷。每天皮下给药增加每种金属的剂量，从低无毒剂量开始，黑麦比较了野生型和MT-null小鼠之间每种金属的累积中位致死剂量（LD50）。野生型小鼠对Cd的LD50比MT-null小鼠高6.9倍。野生型小鼠Zn的LD50比MT-null小鼠高2.4倍，Cu和As的LD50比MT-null小鼠高1.4倍。野生型小鼠Hg的LD50比MT-null小鼠高1.3倍，但差异无统计学意义。野生型和MT-null小鼠在给予Pb和Fe后LD50值无差异。这些结果表明，MT是细胞防御Cd毒性酸致死性的重要蛋白质，但对其他金属致死性的保护作用要小得多。(C) 2001爱思唯尔科学爱尔兰有限公司版权所有。
Metallothionein (MT) is a small-molecular weight. cysteine-rich protein that binds metals. The protective role of MT in Cd toxicity is well established but its ability to protect against toxicity of other metals remains unclear. In this study, wild-type and MT-I and -II null mice (MT-null mice) were used to determine whether MT is protective against the lethality of not only Cd but also Zn, Cu, Fe, Pb. Hg and As. Following daily subcutaneous administration of an increasing dose of each metal, starting with a low, non-toxic dose, rye compared the cumulative median lethal dose (LD50) of each metal between wild-type and MT-null mice. The LD50 of Cd for wild-type mice was 6.9-fold higher than for MT-null mice. The LD50 of Zn was 2.4-fold higher for wild-type mice than for MT-null mice, and 1.4-fold higher for Cu and As. The LD50 of Hg was 1.3-fold higher for wild-type mice than for MT-null mice, but this was not statistically significant. No difference in LD50 values was observed between wild-type and MT-null mice following Pb and Fe administration. These results suggest that MT is an important protein in the cellular defense against Cd toxicity acid lethality, but it provides much less protection against the lethality of the other metals. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.