Short Report: Preliminary Investigation of Trypanosomosis in Exotic Dog Breeds from Zambia's Luangwa and Zambezi Valleys Using LAMP
Short Report: Preliminary Investigation of Trypanosomosis in Exotic Dog Breeds from Zambia's Luangwa and Zambezi Valleys Using LAMP
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DOI：
10.4269/ajtmh.13-0078
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发表时间：
2013-07-01
影响因子：
3.3
通讯作者：
Sugimoto, Chihiro
中科院分区：
文献类型：
--
作者：
Namangala, Boniface;Oparaocha, Elizabeth;Sugimoto, Chihiro
犬非洲锥虫病(CAT)在文献中很少有报道。在这项初步研究中，我们评估了环介导的等温扩增(LAMP)相对于显微镜的性能，以检测来自赞比亚南卢安瓜国家公园和恰瓦狩猎管理区的6个自然感染锥虫的外来犬种的CAT。据我们所知，这是CAT在赞比亚的第一次报告。患者表现出各种非特异性的临床症状。LAMP不仅确认了2010年4月至2012年1月期间被动发现的所有6例寄生虫学阳性猫病例，而且对锥虫物种形成也至关重要。根据LAMP的说法，大多数狗都患有单溶血性感染，要么感染了贡古氏锥虫，要么感染了罗德赛布氏锥虫。因此，LAMP是流行地区诊断锥虫的一种潜在的、简单且经济有效的工具。赞比亚狗中人畜共患锥虫的罕见报告具有公共卫生影响，并证明了对猫的进一步调查是合理的。
Canine African trypanosomosis (CAT) is rarely reported in the literature. In this preliminary study, we evaluated the performance of loop-mediated isothermal amplification (LAMP) against microscopy to detect CAT in six exotic dog breeds naturally infected with trypanosomes from Zambia's South Luangwa National Park and Chiawa Game Management Area. To our knowledge, this is the first report of CAT in Zambia. The patients exhibited a variety of aspecific clinical signs. The LAMP did not only confirm all six parasitologically positive CAT cases detected passively between April 2010 and January 2012, but was also critical in trypanosome speciation. According to LAMP, the majority of the dogs had monolytic infections with either Trypanosoma congolense or Trypanosoma brucei rhodesiense. The LAMP is thus a potential simple and cost-effective tool for trypanosome diagnosis in endemic regions. The rare report of zoonotic trypanosomes in dogs in Zambia has public health implications and justifies further investigations of CAT.