Global and Targeted Metabolomics of Synovial Fluid Discovers Special Osteoarthritis Metabolites
Global and Targeted Metabolomics of Synovial Fluid Discovers Special Osteoarthritis Metabolites
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滑液的整体和靶向代谢组学发现了特殊的骨关节炎代谢物
DOI：
10.1002/jor.23482
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发表时间：
2017-09-01
影响因子：
2.8
通讯作者：
Yang, Xinyu
中科院分区：
文献类型：
--
作者：
Zheng, Kaidi;Shen, Nianhan;Yang, Xinyu
应用代谢组学方法鉴定骨关节炎（OA）滑液中的特异代谢物。采用GC-TOF/MS法检测35例患者（25例OA患者，10例对照者）关节液中的蛋白质含量，并对各指标进行多因素分析。通过VIP值（VIP>1）、Student t检验（p1.5）筛选不同代谢物，并通过LC/MS与24例OA患者和11例对照者滑液中的标准代谢物进行验证，采用受试者工作特征（ROC）分析不同代谢物的分类性能。结果表明，6种不同的代谢产物（谷氨酰胺，1，5-脱水葡萄糖醇，葡萄糖酸内酯，酪胺，苏氨酸和8-氨基辛酸）与OA在全球代谢组学中密切相关。前三种代谢物的验证结果与靶向代谢组学鉴定结果相同。ROC曲线分析表明，关节液中的药物浓度与骨关节炎密切相关。此外，葡萄糖酸内酯浓度在OA和RA之间存在显著差异。水平改变的代谢产物可能是OA发病机制的贡献者，并可用作OA的潜在诊断标准。葡萄糖酸内酯可能成为OA与RA鉴别诊断的新标准。(C)2017骨科研究学会。出版社：Wiley Periodicals，Inc.
To identify special metabolites in synovial fluid of osteoarthritis (OA) via a metabolomics approach. Synovial fluid of 35 participants (25 OA patients and 10 controls) was detected by GC-TOF/MS and multivariate data analysis was applied to analyze correlation among the observations. Different metabolites were screened by VIP value (VIP>1), student t-test (p1.5), and verified with the standard metabolites in the synovial fluid of 24 OA patients and 11 controls by LC/MS. The classification performance of different metabolites was analyzed by receiver operating characteristic (ROC) analysis. The results showed that six different metabolites (glutamine, 1,5-anhydroglucitol, gluconic lactone, tyramine, threonine, and 8-aminocaprylic acid) were strongly associated with OA in global metabolomics. Verified results of the first three metabolites were the same as the identified results using targeted metabolomics. ROC curve analysis demonstrated that their concentrations in synovial fluid were strongly correlated to OA. In addition, the concentrations of gluconic lactone were significantly different between OA and RA. Metabolites with altered levels may be contributors to OA pathogenesis and can be used as potential diagnosis criteria for OA. Gluconic lactone may prove to be a novel criterion for differential diagnosis of OA from RA. (C) 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.