Vitamin D deficiency aggravates diabetes-induced muscle wasting in female mice
Vitamin D deficiency aggravates diabetes-induced muscle wasting in female mice
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维生素 D 缺乏会加剧雌性小鼠糖尿病引起的肌肉萎缩
DOI：
10.1007/s13340-016-0278-7
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发表时间：
2017
影响因子：
2.2
通讯作者：
H. Kaji
中科院分区：
文献类型：
--
作者：
Yukinori Tamura;Haruko Fujito;N. Kawao;H. Kaji
我们最近报道了维生素D缺乏会加重小鼠的糖尿病性骨丢失。虽然维生素D影响肌肉和骨骼，但维生素D状态在糖尿病肌肉损失和肌肉-骨骼关系中的作用仍不清楚。在本研究中，我们研究了维生素D缺乏对糖尿病雌性小鼠肌肉质量，肌肉分化和肌肉来源的体液因子的影响。在用正常或维生素D缺乏的饮食喂养6周后，通过链脲佐菌素（STZ）注射在小鼠中诱导糖尿病。定量计算机断层扫描分析显示，糖尿病小鼠胫骨肌肉质量显着减少，与对照组小鼠相比，诱导糖尿病后4周。维生素D缺乏会加速糖尿病小鼠的肌肉损失。维生素D缺乏加剧了糖尿病诱导的小鼠腓肠肌Pax 7 mRNA水平的下降以及肌肉环指蛋白-1和atrogin-1 mRNA水平的增加。此外，维生素D缺乏降低糖尿病小鼠肌肉中胰岛素样生长因子-1，成纤维细胞生长因子-2和骨甘氨酸的mRNA水平。总之，我们证明了维生素D缺乏会加重糖尿病引起的雌性小鼠肌肉损失。维生素D可能通过糖尿病状态下的肌肉-骨骼关系对肌肉骨骼系统的维持发挥重要作用。
We recently reported that vitamin D deficiency aggravates diabetic bone loss in mice. Although vitamin D affects both muscle and bone, the role of the vitamin D state in diabetic muscle loss and muscle-bone relationships remains unclear. In the present study, we examined the effects of vitamin D deficiency on muscle mass, muscle differentiation and muscle-derived humoral factors linking muscle to bone in diabetic female mice. Diabetes was induced in mice by streptozotocin (STZ) injection after feeding with a normal or vitamin D-deficient diet for 6 weeks. Quantitative computed tomography analysis showed that tibial muscle mass was significantly decreased in diabetic mice compared with control mice 4 weeks after induction of diabetes. Vitamin D deficiency accelerated muscle loss in diabetic mice. Vitamin D deficiency augmented the decreases in Pax7 mRNA levels and the increases in muscle RING-Finger Protein-1 and atrogin-1 mRNA levels induced by diabetes in the gastrocnemius muscle of mice. Moreover, vitamin D deficiency decreased the mRNA levels of insulin-like growth factor-1, fibroblast growth factor-2 and osteoglycin in muscle of diabetic mice. In conclusion, we demonstrated that vitamin D deficiency aggravates muscle loss induced by diabetes in female mice. Vitamin D may exert significant effects on the maintenance of the musculoskeletal system partly through the muscle-bone relationships in diabetic state.
影响因子：
4.8
作者：
Garcia, Leah A.;King, Keisha K.;Artaza, Jorge N.
通讯作者：
Artaza, Jorge N.