Phosphatase and microbial activity with biochemical indicators in semi-permafrost active layer sediments over the past 10,000 years
Phosphatase and microbial activity with biochemical indicators in semi-permafrost active layer sediments over the past 10,000 years
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DOI：
10.1016/j.apgeochem.2005.08.003
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发表时间：
2006-01-01
影响因子：
3.4
通讯作者：
Kobayashi, K
中科院分区：
文献类型：
--
作者：
Takano, Y;Mori, H;Kobayashi, K
对日本北海道陆别地区0-300 cm深度的北方陆地沉积物岩芯样品进行了碱性和酸性磷酸酶活性分析。碱性磷酸酶（ALP）和酸性磷酸酶（ACP）的活性在表层最高，随深度增加而降低，在表层5 cm内，ALP和ACP的活性分别为25.5和22.0 mnol min（-1）g（-1）。这些生物指标与微生物细胞密度和化学指标，包括总有机碳（TOC）和总水解氨基酸（THAA）的测量进行了比较。ALP和ACP与微生物细胞密度的积差相关系数（r）分别为0.949和0.810。THAA和TOC与ALP的相关系数分别为0.997和0.995。酶活性的垂直分布与沉积物中微生物生物量剖面和成岩有机质的分布高度一致。而PO_4浓度的垂直分布与ALP和ACP呈负相关，相关系数分别为-0.937和-0.855。因此，ALP和ACP被证明是有用的生物标志物的微生物活动在陆别，北海道，日本在过去的10 ka。(c)2005爱思唯尔有限公司保留所有权利。
Core samples of boreal terrestrial sediments from depths of 0-300 cm at Rikubetsu, Hokkaido, Japan were analyzed for alkaline and acid phosphatase enzymatic activities. Enzymatic activities of alkaline phosphatase (ALP) and acid phosphatase (ACP) were greatest at the surface and decreased with depth; ALP and ACP activities were 25.5 and 22.0 mnol min(-1) g(-1) respectively, within the top 5 cm. These biological indicators were compared with measurements of microbial cell density and chemical indicators, including total organic C (TOC) and total hydrolyzed amino acids (THAA). The product-moment correlation coefficients (r) for ALP and ACP versus microbial cell density were 0.949 and 0.810, respectively. The coefficients for THAA and TOC versus ALP were 0.997 and 0.995, respectively. Vertical distributions of enzymatic activity are highly consistent with the observed microbial biomass profile and diagenetic organic matter in the sediment. However, the vertical profile Of PO4 concentration shows a negative correlation coefficient for ALP and ACP of -0.937 and -0.855, respectively. Consequently, ALP and ACP were shown to be useful biomarkers of microbial activities in the terrestrial sediment over the past 10 ka at Rikubetsu, Hokkaido, Japan. (c) 2005 Elsevier Ltd. All rights reserved.