Dependence of SARS-CoV-2 (COVID-19) inactivation ability on the crystallinity level of transparent Cu<sub>2</sub>O thin films
Dependence of SARS-CoV-2 (COVID-19) inactivation ability on the crystallinity level of transparent Cu<sub>2</sub>O thin films
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SARS-CoV-2 (COVID-19) 灭活能力对透明 Cu<sub>2</sub>O 薄膜结晶度的依赖性
DOI：
10.1142/s1793604722510535
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发表时间：
2022
影响因子：
1.3
通讯作者：
Sato Mitsunobu
中科院分区：
文献类型：
--
作者：
Wu Hsiang-Jung;Douura Takashi;Kumagai Kyoko;Nagai Hiroki;Kawakami Hiroshi;Nakayama Emi E.;Shioda Tatsuo;Sato Mitsunobu
在无钠玻璃衬底上制备了两种结晶度不同的Cu2O透明薄膜，研究了两种不同结晶度的透明Cu2O薄膜对新冠肺炎的灭活能力。低结晶度的Cu2O薄膜是在室温下以6.72mW的紫外光照射到含有铜络合物的旋涂前驱膜上制备的，孵育1h后，显示出出色的新冠肺炎灭活能力，对病毒的杀灭率为99.999%。经紫外光照射后的薄膜的X射线衍射结果表明，薄膜为立方Cu2O晶格，微晶尺寸为2±1 nm。相反，通过加热含有另一种铜配合物的旋涂前驱体薄膜得到的结晶尺寸为16±3 nm的高结晶度Cu2O薄膜，在与无钠玻璃衬底相同的水平上显示出可以忽略不计的低失活活性。分析了两种Cu2O薄膜在Dulbecco‘s Modify Eagle’s Media(DMEM)中浸泡0.25~2 h后的铜离子洗脱浓度，紫外光辐照薄膜在DMEM浸泡1 h后的淋洗浓度为1.16 ppm，而热处理薄膜的淋洗浓度为0.04 ppm。这表明，Cu2O薄膜上病毒灭活的一个重要因素与介质中从表面发生的铜离子的洗脱密切相关。
The inactivation ability of SARS-CoV-2 (COVID-19) was examined using two types of transparent Cu2O thin films with different crystallinities on a Na-free glass substrate. The low-crystallinity Cu2O thin film, which was fabricated by irradiating 254 nm ultraviolet (UV)-light with an intensity of 6.72 mW cmonto a spin-coated precursor film involving Cucomplexes at room temperature, exhibited an outstanding COVID-19 inactivation ability to reduce 99.999% of the virus after 1 h of incubation. The X-ray diffraction results of the UV-irradiated thin film indicated a cubic Cu2O lattice with a small crystallite size of 2 ± 1 nm. Conversely, the high-crystallinity Cu2O thin film with a crystallite size of 16 ± 3 nm, obtained by heating a spin-coated precursor film containing another Cucomplex, showed a negligibly low inactivation activity at the same level as the Na-free glass substrate. The eluted concentrations of Cu ions from both Cu2O thin films were analyzed after immersion in Dulbecco’s modified Eagle’s medium (DMEM) for 0.25–2 h. The eluted Cu–ion concentration of 1.16 ppm was observed for the UV-irradiated thin film by DMEM immersion after 1 h, but that of 0.04 ppm was observed for the heat-treated thin film. This indicated that an important factor of virus inactivation on Cu2O thin films is highly related to the elution of Cu ions that occurred from the surface in the medium.