Low expression of RBMS3 and SFRP1 are associated with poor prognosis in patients with gastric cancer.
Low expression of RBMS3 and SFRP1 are associated with poor prognosis in patients with gastric cancer.
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RBMS3和SFRP1的低表达与胃癌患者的不良预后相关。
DOI：
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发表时间：
2016-11
影响因子：
5.3
通讯作者：
Li Yongxiang
中科院分区：
文献类型：
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作者：
Zhang Tao;Wu Youliang;Fang Zheng;Yan Qiang;Zhang Shangxin;Sun Ruochuan;Khaliq Junaid;Li Yongxiang
RNA结合基序，单链相互作用蛋白3（RBMS 3）已被报道为肿瘤抑制基因（TSG）在一些鳞状细胞癌。然而，其在胃癌（GC）中的表达水平和临床意义尚不清楚。分泌型卷曲相关蛋白1（SFRP 1）通过抑制Wnt/β-catenin通路在多种肿瘤中发挥抑癌作用。然而，其在胃癌中的表达水平和临床意义仍存在争议。本研究采用实时荧光定量PCR和Western Blot方法检测了23例新鲜胃癌组织及相应正常组织中RBMS 3和SFRP 1的mRNA和蛋白水平。应用免疫组化方法检测172例胃癌组织芯片中RBMS 3和SFRP 1的表达水平。结果发现69.57%（16/23）和73.91%（17/23）的胃癌组织RBMS 3 mRNA和蛋白表达水平均显著低于正常组织。同样，78.26%（18/23）和65.22%（15/23）的GC组织在mRNA和蛋白水平上表达低于相应的正常组织。RBMS 3和SFRP 1蛋白的低表达与肿瘤的病理分级和预后有关（P均<0.05）。多因素分析显示RBMS 3和SFRP 1共表达状态是胃癌患者的独立预后因素。最后，RBMS 3和SFRP 1的表达水平（mRNA和蛋白质）之间的正相关性。总体而言，RBMS 3和SFRP 1在GC中均异常低表达，RBMS 3和SFRP 1共表达是GC的潜在预后预测因子。
RNA binding motif, single stranded interacting protein 3 (RBMS3) has been reported as a tumor suppressor gene (TSG) in some squamous carcinoma. However, its expression levels and clinical significance in gastric cancer (GC) remains unclear. Secreted frizzled-related protein 1 (SFRP1) plays a role of tumor suppressor in many cancers by inhibiting Wnt/β-catenin pathway. Nevertheless, its expression levels and clinical significance in GC are in dispute. In this study, quantitative real-time PCR and Western Blot were used to measure the mRNA and protein level of RBMS3 and SFRP1 in 23 fresh GC and corresponding normal tissues. Immunohistochemistry assay was performed to further measure the protein level of RBMS3 and SFRP1 on population-based tissue microarrays consisting of 172 GC cases. We found that 69.57% (16/23) and 73.91% (17/23) GC tissues expressed remarkably lower RBMS3 than the matched normal tissues respectively in mRNA and protein levels. Similarly, 78.26% (18/23) and 65.22% (15/23) GC tissues expressed lower SFRP1 than the matched normal tissues respectively in mRNA and protein levels. Additionally, the low expression of RBMS3 and SFRP1 protein were all significantly related to the poor histological grades and prognosis (all P<0.05). In multivariate analysis, RBMS3 and SFRP1 co-expression status was independent prognostic factor for GC patients. Finally, the positive correlation between expression levels (mRNA and protein) of RBMS3 and SFRP1 was observed. Overall, RBMS3 and SFRP1 are both aberrantly low expressed in GC, and RBMS3 and SFRP1 co-expression is a potential prognosis predictor of GC.