Changes in hepatic messenger RNA for phosphoenolpyruvate carboxykinase (GTP) during development.
Changes in hepatic messenger RNA for phosphoenolpyruvate carboxykinase (GTP) during development.
复制标题
发育过程中磷酸烯醇丙酮酸羧激酶 (GTP) 肝脏信使 RNA 的变化。
DOI：
--
复制
发表时间：
1978
影响因子：
11.1
通讯作者：
R. Hanson
中科院分区：
文献类型：
--
作者：
J. Ruiz;R. Ingram;R. Hanson
磷酸烯醇式丙酮酸羧激酶（GTP）[GTP;草酰乙酸羧裂解酶（转磷酸化）; EC 4.1.1.32]在胎儿期大鼠肝细胞质中不存在，最初在出生时合成。通过在子宫内向胎儿动物注射双丁酰环AMP或胰高血糖素，可过早诱导该酶的从头合成。在这项研究中，小麦胚芽翻译分析用于定量磷酸烯醇式丙酮酸羧激酶的总功能mRNA的水平在孕21天的胎鼠在不同的诱导情况下的肝脏。在胎鼠肝脏中可检测到该酶的可翻译mRNA。无论是胰高血糖素或双丁酰环AMP在子宫内的胎鼠引起显着的诱导功能的mRNA这种酶。二丁酰环磷酸腺苷给药后3小时，可翻译的mRNA水平增加了近23倍，但到6小时，水平下降约60%。在21天的胎鼠中，在给予双丁酰环腺苷酸之前给予放线菌素D，可防止细胞质中新合成的含poly（A）的RNA的出现，以及磷酸烯醇丙酮酸羧激酶的可翻译mRNA的诱导。在过早分娩并维持不同时间的动物中，酶的可翻译mRNA以与酶合成所观察到的速率相当的速率在肝脏中积累。
Phosphoenolpyruvate carboxykinase (GTP) [GTP;oxaloacetate carboxy-lyase(transphosphorylating); EC 4.1.1.32] is absent in rat liver cytosol during fetal life and is synthesized initially at birth. De novo synthesis of the enzyme can be induced prematurely by injection of dibutyryl cyclic AMP or glucagon into fetal animals in utero. In this study a wheat germ translation assay was used to quantitate the level of total functional mRNA for phosphoenolpyruvate carboxykinase in the liver of fetal rats at 21 days of pregnancy under different induction situations. The translatable mRNA for the enzyme was marginally detectable in fetal rat liver. Administration of either glucagon or dibutyryl cyclic AMP to fetal rats in utero caused a marked induction of functional mRNA for this enzyme. Three hours after administration of dibutyryl cyclic AMP, the level of translatable mRNA increased almost 23-fold, but by 6 hr the level dropped approximately 60%. Administration of actinomycin D prior to dibutyryl cyclic AMP in 21-day fetal rats prevented the appearance of newly synthesized poly(A)-containing RNA in the cytoplasm as well as the induction of translatable mRNA for phosphoenolpyruvate carboxykinase. In animals delivered prematurely and maintained for varying periods, the translatable mRNA for the enzyme accumulated in the liver at a rate comparable to that observed for enzyme synthesis.