Prevalence of the F-type lectin domain
Prevalence of the F-type lectin domain
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DOI：
10.1093/glycob/cwv029
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发表时间：
2015-08-01
期刊：
影响因子：
4.3
通讯作者：
Ramya, T. N. C.
中科院分区：
文献类型：
--
作者：
Bishnoi, Ritika;Khatri, Indu;Ramya, T. N. C.
F型凝集素是具有特征性岩藻糖和钙结合序列基序以及独特的凝集素折叠（“F型”折叠）的岩藻凝集素。F-型凝集素在遗传学上广泛分布，具有选择性分布。几种真核F-型凝集素已被生化和结构表征，并且F-型凝集素结构域（FLD）也已在细菌蛋白、缓症链球菌凝集素和肺炎链球菌SP2159中被研究。然而，有很少的知识的发生程度的FLD及其域组织，特别是在细菌中。我们现在已经挖掘了广泛的基因组序列信息，在公共数据库中的敏感的序列搜索技术，以彻底调查原核和真核FLD。我们报告了437个来自真核生物、真细菌和病毒蛋白质的FLD序列簇（在80%的序列同一性处聚类）。结构域的结构是多样的，但大多数是保守的密切相关的生物体，和结构域组织的细菌FLD含有蛋白质是非常不同的，从他们的真核生物的对应物，这表明独特的专业化的FLD，以适应不同的要求。几个非典型的系统发育协会暗示横向转移。在真核生物中，我们观察到扩大的FLD的发生和域组织多样性的分类群软体动物，Hemichordata和Branchiostomi，也许是巧合，更强调先天免疫策略，在这些生物体。我们在此鉴定的具有不同结构域组织的天然存在的FLD将有助于未来的研究，旨在创建设计者分子平台，用于将所需的生物活性导向靶位中的岩藻糖基化糖缀合物。
F-type lectins are fucolectins with characteristic fucose and calcium-binding sequence motifs and a unique lectin fold (the "F-type" fold). F-type lectins are phylogenetically widespread with selective distribution. Several eukaryotic F-type lectins have been biochemically and structurally characterized, and the F-type lectin domain (FLD) has also been studied in the bacterial proteins, Streptococcus mitis lectinolysin and Streptococcus pneumoniae SP2159. However, there is little knowledge about the extent of occurrence of FLDs and their domain organization, especially, in bacteria. We have now mined the extensive genomic sequence information available in the public databases with sensitive sequence search techniques in order to exhaustively survey prokaryotic and eukaryotic FLDs. We report 437 FLD sequence clusters (clustered at 80% sequence identity) from eukaryotic, eubacterial and viral proteins. Domain architectures are diverse but mostly conserved in closely related organisms, and domain organizations of bacterial FLD-containing proteins are very different from their eukaryotic counterparts, suggesting unique specialization of FLDs to suit different requirements. Several atypical phylogenetic associations hint at lateral transfer. Among eukaryotes, we observe an expansion of FLDs in terms of occurrence and domain organization diversity in the taxa Mollusca, Hemichordata and Branchiostomi, perhaps coinciding with greater emphasis on innate immune strategies in these organisms. The naturally occurring FLDs with diverse domain organizations that we have identified here will be useful for future studies aimed at creating designer molecular platforms for directing desired biological activities to fucosylated glycoconjugates in target niches.