Saw Palmetto Berry Extract inhibits cell growth and Cox-2 expression in prostatic cancer cells
Saw Palmetto Berry Extract inhibits cell growth and Cox-2 expression in prostatic cancer cells
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DOI：
10.1006/cbir.2001.0779
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发表时间：
2001-01-01
影响因子：
3.9
通讯作者：
Sharma, CP
中科院分区：
文献类型：
--
作者：
Goldmann, WH;Sharma, AL;Sharma, CP
锯棕榈浆果提取物 (SPBE) 是缓解良性前列腺增生 (BPH) 症状的常用材料，使用一组前列腺细胞系以纯油形式进行了细胞毒性检查； 267B-1、BRFF-41T 和 LNCaP。当使用 SPBE 给药 3 天时，这些前列腺来源的细胞系的增殖受到不同程度的抑制。对于细胞系267B-1和BRFF-41T，抑制这些细胞系50％生长(IC50)所需的SPBE量为每ml培养基20-30nl当量的SPBE，对于LNCaP细胞系大约多10倍。 SPBE 给药对这些细胞系的影响不是不可逆的，因为用 IC50 浓度的 SPBE 处理 30 分钟不会抑制它们的生长。当在每ml培养基中存在200nl SPBE当量的情况下生长时，正常前列腺细胞被抑制20-25%。其他非前列腺癌细胞系（即 Jurkat 和 HT-29）的生长受到约的影响。分别为 50% 和 40%。当LNCaP细胞在二氢睾酮和SPBE存在下生长时，与在血清和SPBE存在下生长的LNCaP细胞相比，IC50浓度显着降低。 SPBE 处理这些细胞系后细胞生长减少可能与 Cox-2 表达减少有关，并且可能是由于 Bcl-2 表达中观察到的变化所致。由于其组成型表达，在类似条件下Cox-1的表达不受影响。由于 Cox-2 表达增加与前列腺癌发病率增加相关，因此 SPBE 降低其表达将为进一步研究其对抗 BPH 和前列腺癌化学预防的用途提供基础。 (C) 2001 年学术出版社。
The cytotoxicity of a commonly used material to alleviate the symptoms of benign prostatic hyperplasia (BPH), Saw Palmetto Berry Extract (SPBE), was examined as neat oil using a set of prostatic cell lines; 267B-1, BRFF-41T and LNCaP. Proliferation of these prostatic derived cell lines is inhibited to different degrees when dosed for 3 days with SPBE. The amount of SPBE required to inhibit 50% growth (IC50) Of these cell lines was 20-30 nl equivalents of SPBE per ml of medium for cell lines 267B-1 and BRFF-41T and approximately 10-fold more for the LNCaP cell line. The effect of SPBE dosing on these cell lines, is not irreversible, since a 30 min treatment with SPBE at an IC50 concentration does not inhibit their growth. Normal prostate cells were inhibited by 20-25% when grown in the presence of 200 nl SPBE equivalent per ml media. Growth of other non-prostatic cancer cell lines, i.e. Jurkat and HT-29, was affected by approx. 50% and 40%, respectively. When LNCaP cells Were grown in the presence of dihydrotestosterone and SPBE, the IC50 concentration decreased significantly compared to LNCaP cells grown in the presence of serum and SPBE. Reduced cellular growth after SPBE treatment of these cell lines may relate to decreased expression of Cox-2 and may be due to changes observed in the expression of Bcl-2. Expression of Cox-l under similar conditions is not affected because of its constitutive expression. Since increased Cox-2 expression is associated with an increased incidence of prostate cancer, and decrease in its expression by SPBE would provide a basis for further investigation of its use against BPH and in prostatic cancer chemoprevention. (C) 2001 Academic Press.