Guidelines for an optimized differential centrifugation of cells.
Guidelines for an optimized differential centrifugation of cells.
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DOI：
10.1016/j.bbrep.2023.101585
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发表时间：
2023-12
影响因子：
2.7
通讯作者：
中科院分区：
文献类型：
--
作者：
文献综述揭示了一个显着的缺陷，在概念上的理解有关离心，在医疗和研究方案的关键步骤。离心力的任意波动对结果的再现性构成潜在威胁。为了解决这个问题，我们提出了简洁的指南，整合了温度，渗透压，液体体积和粘度等关键因素。这些指南旨在提高对细胞悬浮液最佳沉降条件的理解。此外，我们介绍了一个标准化的协议，用于确定最佳的RCF和离心时间。其目标是最大限度地提高沉降效率，同时最大限度地减少细胞损伤，为离心实践中的普遍适用和可重复方法做出贡献。缺乏对离心的概念性理解会危及医疗和研究程序中的结果再现性。温度、渗透压、液体体积和粘度在实现细胞的最佳沉降中起着至关重要的作用。需要一种利用离心力来获得最佳细胞沉降的有效方案。
Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose concise guidelines that integrate key factors such as temperature, osmolarity, fluid volume, and viscosity. These guidelines aim to enhance comprehension of optimal sedimentation conditions for cell suspensions. Additionally, we introduce a standardized protocol for determining the optimal RCF and centrifugation time. The goal is to maximize sedimentation efficiency while minimizing cell damage, contributing to a universally applicable and reproducible method in centrifugation practices. Lack of conceptual understanding of centrifugation endangers result reproducibility in medical and research procedures. Temperature, osmolarity, fluid volume, and viscosity play crucial roles in achieving optimal sedimentation of cells. An effective protocol for utilizing centrifugal force to attain optimal cell sedimentation is required.