Comparison of articular cartilage images assessed by high-frequency ultrasound microscope and scanning acoustic microscope
Comparison of articular cartilage images assessed by high-frequency ultrasound microscope and scanning acoustic microscope
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DOI：
10.1007/s00264-011-1263-1
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Itoi, Eiji
中科院分区：
文献类型：
--
作者：
Hagiwara, Yoshihiro;Saijo, Yoshifumi;Itoi, Eiji
本研究的目的是比较新开发的高频超声成像系统(HFUIS)和扫描声学显微镜(SAM)的图像，并计算它们的皮尔逊积矩相关性，以期将高频超声成像系统应用于临床。标本浸泡在生理盐水中，用高频超声扫描仪进行扫描。HFUIS的强度通过相同距离的钢板反射来归一化。扫描后，标本用多聚甲醛固定，脱钙，石蜡包埋。制备薄层组织，用于SAM评价。扫描结束后，对关节软骨的浅、中、深三层进行独立评价并计算其相互关系，其中浅层和深层相对信号较高，中层相对信号不均匀。高频超声的高相对强度与扫描电子显微镜的高声速区有很强的相关性(皮尔逊积矩相关，浅层0.704，中层0.731)。高频超声产生了关节软骨的高分辨率图像，其强度与扫描电子显微镜的声速有很强的相关性。
The purpose of this study was to compare images of a newly developed high-frequency ultrasound imaging system (HFUIS) and scanning acoustic microscope (SAM) and to calculate their Pearson product moment correlations with a view to applying HFUIS for clinical use.Cylindrical cartilage-bone complexes from adult male Sprague-Dawley rats were obtained. The specimens were immersed in normal saline and scanned by HFUIS. Intensity by HFUIS was normalised by reflection from a steel plate at the same distance. After the scanning, specimens were fixed with paraformaldehyde, decalcified and embedded in paraffin. Thinly sliced tissues were prepared for SAM evaluation. After the scanning, three layers of articular cartilage (superficial, middle and deep) were independently evaluated and their relationships calculated.The superficial and deep layers indicated high relative intensity, whereas the middle layer showed nonhomogeneous relative intensity by HFUIS. A high relative intensity by HFUIS and high sound speed area by SAM had strong correlations (Pearson product moment correlation, superficial layer 0.704, middle layer 0.731).HFUIS produced high-resolution images of the articular cartilage and its intensity was strongly correlated with sound speed by SAM.